Tyler F, Fisher D, d’Ettorre P, Rodríguez-Muñoz R, Tregenza T (2015). Chemical cues mediate 
species recognition in field crickets.  Frontiers in Ecology & Evolution – Chemical Ecology. 
DOI: 10.3389/fevo.2015.00048

Tyler et al Frontiers15 Gryllus CHC Peak Areas
Relative peak sizes of each of the 44 CHCs, for each individual cricket.  CHCs are listed in 
order of retention time.  Relative peak size was calculated by dividing the peak area of a 
given compound by the peak area of the internal standard in that sample.  Sex (‘female’ or 
‘male’) and species (‘G.bimac’ or ‘G.camp’) are given for each cricket.

Tyler et al Frontiers15 Aggression to Heterospecifics
Level of aggression expressed by G. campestris females towards G. bimaculatus males prior 
to and after antennal treatment.  Antennae were either treated with a control (‘Control’) or 
chemically ablated (‘Zinc sulphate’).  Aggression was recorded as seconds spent performing 
aggressive behavior before and after treatment (‘Pre Seconds’ and ‘Post Seconds’) and as 
number of mandible flares (‘Pre Flares’ and ‘Post Flares’). Change in aggression was 
calculated as the aggression value in the post-treatment trial, minus the aggression value in 
the pre-treatment trial (‘Change Seconds’ or ‘Change Flares’).