LOCUS       Exported                8789 bp ds-DNA     circular SYN 03-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    NO-CAR-pNIC28
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Will
  TITLE     Direct Submission
  JOURNAL   Exported 23 Sep 2016 from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        1..19
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             89..3628
                     /codon_start=1
                     /note="noCAR all"
                     /translation="MHHHHHHSSGVDLGTENLYFQSMLDDARAERRERRIADALADDQV
                     REAAADAAVSESVRRVEVRLARIVDAVMSGYGDRAALAWRRSELVDGAVRLLPEYSTMT
                     YRELWRQAGAVAAEWGADAESPVRAEDFVCTLGFTSPDYTVVDLALMRLAAVAVPLQAS
                     ASVAQWRSIMAETEPRMLAASAETLPAAVEAVLGGFAPRRVLVFDYRPELEAHRSAVDS
                     ARERLAEVGCTVATVADAVDRGANLPAPLRIPSDRERLALLIYTSGSTGAPKGAMYTDR
                     LVAGLWLSANEIRVPALTMNYMPLSHIAGRMSLYGTLMRGGTAYFAAASDMSTLLDDFG
                     LARPTELFLVPRVCELLHQRYQSELDRRVVAGEDAETAATNVKAELRERVLGGRYLTAL
                     SGSAPLAAEMKTFMESLLDDELHDGYGSTEAGGSVLLDNRIKRPPVLDYRLVDVPELGY
                     FRTDKPHPRGELLLKTESMFPGYYKRPEITAEMFDADGFYRTGDVVAELGPEQLVYVDR
                     RNNVLKLSQGEFVTVAALEAVYATSPLIRQIFVYGSSERAYLLAVVVPTDAVLALPAAR
                     ARAEVSESLQRIAKESGLRPYEIPRDLIIESEPFTIDNGLLSGIGKLLRPKLKEHYGER
                     LEQLYAELAEQREDELTALRRGAHDRPILDTVTRAAGAVLDLTAGEVSPDAHFADLGGD
                     SLSALSFSTLLRDIFGVEVPVGFIVGPATDLARIAEYLVSERDSGSRPTAATVHGDDGL
                     LRADDLALEAFLDPATLDAAAHLPSALEPPRTVLLTGANGYLGRFLALEWLQRLDVSGG
                     TLICLIRGSDADSARRRLDAVFATGDPELEAHYRELAERRLRVLPGDIGEPNLGLREQD
                     WRDLAETVDLIVHPAALVNHVLPYAQLFGPNVVGTAEVIRLALTSRLKPVTYLSTVAVS
                     AGIDPETFTEDGDIREISPVRRLDDGYANGYGNSKWAGEVLLRNAHDRFGLPVAVFRSD
                     MILAHSRYAGQLNVPDMFTRLLLSVLATGLAPGSFHDAHGERHRAHYDGLPADFTAAAV
                     TTLGSRVTSGYETYDVLNPHDDGISLDTFVDWLIEAGHPIDRIDDYAEWFARFDTALRA
                     LPEHQRQHSLLPLLHAYRRPTPPLHGVALPAKHFRAAVQQAKLGPDGDIPHVTRELIEK
                     YASDLRLLGLIQG"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /note="TEV site"
                     /translation="ENLYFQS"
     CDS             155..3628
                     /codon_start=1
                     /note="Nocardia otitidiscaviarum"
                     /translation="MLDDARAERRERRIADALADDQVREAAADAAVSESVRRVEVRLAR
                     IVDAVMSGYGDRAALAWRRSELVDGAVRLLPEYSTMTYRELWRQAGAVAAEWGADAESP
                     VRAEDFVCTLGFTSPDYTVVDLALMRLAAVAVPLQASASVAQWRSIMAETEPRMLAASA
                     ETLPAAVEAVLGGFAPRRVLVFDYRPELEAHRSAVDSARERLAEVGCTVATVADAVDRG
                     ANLPAPLRIPSDRERLALLIYTSGSTGAPKGAMYTDRLVAGLWLSANEIRVPALTMNYM
                     PLSHIAGRMSLYGTLMRGGTAYFAAASDMSTLLDDFGLARPTELFLVPRVCELLHQRYQ
                     SELDRRVVAGEDAETAATNVKAELRERVLGGRYLTALSGSAPLAAEMKTFMESLLDDEL
                     HDGYGSTEAGGSVLLDNRIKRPPVLDYRLVDVPELGYFRTDKPHPRGELLLKTESMFPG
                     YYKRPEITAEMFDADGFYRTGDVVAELGPEQLVYVDRRNNVLKLSQGEFVTVAALEAVY
                     ATSPLIRQIFVYGSSERAYLLAVVVPTDAVLALPAARARAEVSESLQRIAKESGLRPYE
                     IPRDLIIESEPFTIDNGLLSGIGKLLRPKLKEHYGERLEQLYAELAEQREDELTALRRG
                     AHDRPILDTVTRAAGAVLDLTAGEVSPDAHFADLGGDSLSALSFSTLLRDIFGVEVPVG
                     FIVGPATDLARIAEYLVSERDSGSRPTAATVHGDDGLLRADDLALEAFLDPATLDAAAH
                     LPSALEPPRTVLLTGANGYLGRFLALEWLQRLDVSGGTLICLIRGSDADSARRRLDAVF
                     ATGDPELEAHYRELAERRLRVLPGDIGEPNLGLREQDWRDLAETVDLIVHPAALVNHVL
                     PYAQLFGPNVVGTAEVIRLALTSRLKPVTYLSTVAVSAGIDPETFTEDGDIREISPVRR
                     LDDGYANGYGNSKWAGEVLLRNAHDRFGLPVAVFRSDMILAHSRYAGQLNVPDMFTRLL
                     LSVLATGLAPGSFHDAHGERHRAHYDGLPADFTAAAVTTLGSRVTSGYETYDVLNPHDD
                     GISLDTFVDWLIEAGHPIDRIDDYAEWFARFDTALRALPEHQRQHSLLPLLHAYRRPTP
                     PLHGVALPAKHFRAAVQQAKLGPDGDIPHVTRELIEKYASDLRLLGLIQG"
     CDS             3650..3667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      3734..3781
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3818..4273
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(4366..5181)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5303..5891
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6077..6219
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6321..6512)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7321..8403)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(8404..8481)
                     /gene="lacI"
                     /note="lacI promoter"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt
      121 agatctgggt accgagaacc tgtacttcca atccatgctg gacgacgccc gtgccgaacg
      181 ccgtgaacgc cgtattgctg acgctctggc cgacgaccaa gttcgcgaag ccgctgctga
      241 cgcggccgtt agtgaatccg tccgtcgcgt cgaagtgcgt ctggcccgta tcgtggatgc
      301 cgttatgagt ggctatggtg accgtgcagc tctggcctgg cgtcgctccg aactggtgga
      361 tggtgcagtt cgtctgctgc cggaatatag caccatgacg taccgcgaac tgtggcgtca
      421 ggcgggtgca gttgccgcag aatggggcgc tgatgccgaa agcccggttc gtgccgaaga
      481 ctttgtctgc accctgggtt tcacgtctcc ggattatacc gtggttgacc tggcgctgat
      541 gcgtctggca gctgttgcgg tcccgctgca agcctcagca tcggtggcgc aatggcgctc
      601 aattatggcc gaaaccgaac cgcgcatgct ggccgcaagc gccgaaaccc tgccggcagc
      661 tgttgaagca gtgctgggcg gttttgctcc gcgtcgcgtg ctggttttcg attatcgccc
      721 ggaactggaa gcccatcgta gtgcagtgga ctccgctcgc gaacgtctgg cagaagttgg
      781 ctgtaccgtt gctaccgttg ctgatgccgt ggatcgcggt gcgaacctgc cggccccgct
      841 gcgtatcccg agtgatcgcg aacgtctggc gctgctgatt tataccagcg gctctacggg
      901 tgcaccgaaa ggtgctatgt acaccgatcg cctggtcgcg ggtctgtggc tgtccgcaaa
      961 cgaaatccgt gtgccggccc tgacgatgaa ttatatgccg ctgagccaca ttgcaggtcg
     1021 catgtctctg tatggcaccc tgatgcgtgg cggtaccgcc tactttgccg cagcaagcga
     1081 tatgtctacc ctgctggatg attttggtct ggcacgtccg accgaactgt ttctggtgcc
     1141 gcgcgtttgc gaactgctgc atcagcgtta tcaaagcgaa ctggatcgtc gcgttgttgc
     1201 cggtgaagat gcggaaaccg ctgccacgaa cgttaaagcc gaactgcgcg aacgtgtcct
     1261 gggcggtcgt tatctgaccg cactgagtgg ttccgctccg ctggcagcag aaatgaaaac
     1321 gtttatggaa agcctgctgg atgacgaact gcacgatggc tacggttcaa ccgaagcggg
     1381 cggttcggtc ctgctggata atcgcatcaa acgtccgccg gtgctggatt atcgcctggt
     1441 cgacgtgccg gaactgggtt acttccgcac cgataaaccg catccgcgtg gcgaactgct
     1501 gctgaaaacg gaatctatgt ttccgggtta ttacaaacgc ccggaaatta ccgcggaaat
     1561 gtttgatgcc gacggtttct atcgtaccgg tgatgttgtt gccgaactgg gtccggaaca
     1621 gctggtttac gtcgatcgtc gcaacaatgt gctgaaactg tcacagggtg aatttgtgac
     1681 cgttgctgcg ctggaagctg tttatgcgac gtcgccgctg attcgccaga tctttgtcta
     1741 tggcagctct gaacgtgcat acctgctggc tgtggttgtc ccgaccgatg cggtgctggc
     1801 actgccggcg gcacgtgcac gtgcagaagt ttcagaatcg ctgcaacgca tcgcgaaaga
     1861 aagcggtctg cgcccgtacg aaattccgcg tgatctgatt atcgaaagcg aaccgttcac
     1921 cattgacaat ggcctgctgt ctggcatcgg taaactgctg cgcccgaaac tgaaagaaca
     1981 ttatggtgaa cgtctggaac agctgtacgc tgaactggcg gaacaacgtg aagatgaact
     2041 gaccgcactg cgtcgcggcg ctcatgatcg tccgattctg gacaccgtga cgcgtgctgc
     2101 gggtgcagtt ctggatctga ccgctggcga agtgagcccg gacgcccatt ttgcagatct
     2161 gggcggtgac agcctgtctg ccctgagttt ttccaccctg ctgcgcgata ttttcggcgt
     2221 cgaagtgccg gttggtttta tcgtgggccc ggcgacggat ctggctcgta ttgcggaata
     2281 tctggtttct gaacgtgact caggttcgcg cccgaccgca gcaaccgtgc atggcgatga
     2341 tggtctgctg cgtgccgatg acctggcact ggaagcgttt ctggacccgg caaccctgga
     2401 tgctgccgca cacctgccga gcgccctgga accgccgcgt accgttctgc tgaccggtgc
     2461 aaacggttat ctgggccgct ttctggcact ggaatggctg caacgtctgg atgtgtctgg
     2521 cggtaccctg atttgtctga ttcgcggctc agatgcggac agcgcccgtc gccgtctgga
     2581 tgcagttttt gcaacgggtg acccggaact ggaagcgcat taccgcgaac tggcggaacg
     2641 ccgtctgcgt gtgctgccgg gtgatatcgg cgaaccgaat ctgggcctgc gcgaacagga
     2701 ttggcgtgac ctggcagaaa ccgtcgatct gattgtgcat ccggcagctc tggtcaacca
     2761 cgtgctgccg tatgcgcaac tgttcggtcc gaatgtggtt ggcaccgcag aagtgatccg
     2821 cctggctctg acgagccgtc tgaaaccggt tacctacctg agtacggtcg ctgtgtccgc
     2881 gggtattgat ccggaaacct ttacggaaga tggcgacatt cgtgaaatca gtccggttcg
     2941 ccgtctggat gacggttatg ccaacggcta cggtaattcc aaatgggcag gcgaagtgct
     3001 gctgcgtaat gcccatgatc gttttggtct gccggttgcg gtcttccgca gtgacatgat
     3061 cctggcgcac tcccgttatg ccggtcagct gaatgttccg gatatgttca cccgtctgct
     3121 gctgtcagtc ctggccaccg gtctggcacc gggctcgttt catgatgcgc acggtgaacg
     3181 ccatcgtgcc cactacgatg gtctgccggc agattttacc gccgcagcag tcaccaccct
     3241 gggtagccgt gtgacctctg gctatgaaac gtacgatgtt ctgaacccgc atgatgacgg
     3301 catctcactg gatacctttg tggactggct gattgaagcg ggtcacccga ttgatcgcat
     3361 cgatgactat gcagaatggt ttgctcgttt tgacaccgca ctgcgtgcac tgccggaaca
     3421 tcagcgtcaa cactccctgc tgccgctgct gcatgcatac cgccgtccga ccccgccgct
     3481 gcatggcgtg gctctgccgg cgaaacactt tcgtgctgcg gttcagcaag cgaaactggg
     3541 tccggatggc gacattccgc atgtgacccg tgaactgatt gaaaaatatg cgagcgacct
     3601 gcgtctgctg ggtctgatcc aaggctgaaa gcttgcggcc gcactcgagc accaccacca
     3661 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     3721 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     3781 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     3841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3961 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4021 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4201 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4261 acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4321 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
     4381 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     4441 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     4501 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     4561 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     4621 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     4681 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     4741 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     4801 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     4861 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     4921 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     4981 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     5041 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     5101 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt
     5161 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     5221 tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6001 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6061 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6121 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6181 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     6241 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6301 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     6361 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     6421 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     6481 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     6541 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     6601 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     6661 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     6721 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     6781 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     6841 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     6901 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     6961 acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     7021 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     7081 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     7141 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     7201 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     7261 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     7321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7381 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     7441 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     7501 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     7561 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     7621 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     7681 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     7741 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     7801 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     7861 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     7921 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     7981 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     8041 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     8101 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     8161 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     8221 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     8281 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     8341 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     8401 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     8461 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     8521 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     8581 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     8641 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     8701 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     8761 agaggatcga gatctcgatc ccgcgaaat
//
