LOCUS       Exported                8878 bp ds-DNA     circular SYN 03-AUG-2016
DEFINITION  Bacterial vector for expression of N-terminally 6xHis-tagged 
            proteins with a thrombin site. For other reading frames, use 
            pET-28b(+) or pET-28c(+).
ACCESSION   .
VERSION     .
KEYWORDS    msCAR in pET28a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 23 Sep 2016 from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /note="lacI promoter"
     promoter        4984..5002
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5058..5063
                     /note="ribosome binding site"
     CDS             5071..8700
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMTSDVHDAT
                     DGVTETALDDEQSTRRIAELYATDPEFAAAAPLPAVVDAAHKPGLRLAEILQTLFTGYG
                     DRPALGYRARELATDEGGRTVTRLLPRFDTLTYAQVWSRVQAVAAALRHNFAQPIYPGD
                     AVATIGFASPDYLTLDLVCAYLGLVSVPLQHNAPVSRLAPILAEVEPRILTVSAEYLDL
                     AVESVRDVNSVSQLVVFDHHPEVDDHRDALARAREQLAGKGIAVTTLDAIADEGAGLPA
                     EPIYTADHDQRLAMILYTSGSTGAPKGAMYTEAMVARLWTMSFITGDPTPVINVNFMPL
                     NHLGGRIPISTAVQNGGTSYFVPESDMSTLFEDLALVRPTELGLVPRVADMLYQHHLAT
                     VDRLVTQGADELTAEKQAGAELREQVLGGRVITGFVSTAPLAAEMRAFLDITLGAHIVD
                     GYGLTETGAVTRDGVIVRPPVIDYKLIDVPELGYFSTDKPYPRGELLVRSQTLTPGYYK
                     RPEVTASVFDRDGYYHTGDVMAETAPDHLVYVDRRNNVLKLAQGEFVAVANLEAVFSGA
                     ALVRQIFVYGNSERSFLLAVVVPTPEALEQYDPAALKAALADSLQRTARDAELQSYEVP
                     ADFIVETEPFSAANGLLSGVGKLLRPNLKDRYGQRLEQMYADIAATQANQLRELRRAAA
                     TQPVIDTLTQAAATILGTGSEVASDAHFTDLGGDSLSALTLSNLLSDFFGFEVPVGTIV
                     NPATNLAQLAQHIEAQRTAGDRRPSFTTVHGADATEIRASELTLDKFIDAETLRAAPGL
                     PKVTTEPRTVLLSGANGWLGRFLTLQWLERLAPVGGTLITIVRGRDDAAARARLTQAYD
                     TDPELSRRFAELADRHLRVVAGDIGDPNLGLTPEIWHRLAAEVDLVVHPAALVNHVLPY
                     RQLFGPNVVGTAEVIKLALTERIKPVTYLSTVSVAMGIPDFEEDGDIRTVSPVRPLDGG
                     YANGYGNSKWAGEVLLREAHDLCGLPVATFRSDMILAHPRYRGQVNVPDMFTRLLLSLL
                     ITGVAPRSFYIGDGERPRAHYPGLTVDFVAEAVTTLGAQQREGYVSYDVMNPHDDGISL
                     DVFVDWLIRAGHPIDRVDDYDDWVRRFETALTALPEKRRAQTVLPLLHAFRAPQAPLRG
                     APEPTEVFHAAVRTAKVGPGDIPHLDEALIDKYIRDLREFGLI"
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             5110..5127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     CDS             5131..5163
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     misc_feature    5167..8721
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             5179..8700
                     /codon_start=1
                     /note="msCAR"
                     /translation="MTSDVHDATDGVTETALDDEQSTRRIAELYATDPEFAAAAPLPAV
                     VDAAHKPGLRLAEILQTLFTGYGDRPALGYRARELATDEGGRTVTRLLPRFDTLTYAQV
                     WSRVQAVAAALRHNFAQPIYPGDAVATIGFASPDYLTLDLVCAYLGLVSVPLQHNAPVS
                     RLAPILAEVEPRILTVSAEYLDLAVESVRDVNSVSQLVVFDHHPEVDDHRDALARAREQ
                     LAGKGIAVTTLDAIADEGAGLPAEPIYTADHDQRLAMILYTSGSTGAPKGAMYTEAMVA
                     RLWTMSFITGDPTPVINVNFMPLNHLGGRIPISTAVQNGGTSYFVPESDMSTLFEDLAL
                     VRPTELGLVPRVADMLYQHHLATVDRLVTQGADELTAEKQAGAELREQVLGGRVITGFV
                     STAPLAAEMRAFLDITLGAHIVDGYGLTETGAVTRDGVIVRPPVIDYKLIDVPELGYFS
                     TDKPYPRGELLVRSQTLTPGYYKRPEVTASVFDRDGYYHTGDVMAETAPDHLVYVDRRN
                     NVLKLAQGEFVAVANLEAVFSGAALVRQIFVYGNSERSFLLAVVVPTPEALEQYDPAAL
                     KAALADSLQRTARDAELQSYEVPADFIVETEPFSAANGLLSGVGKLLRPNLKDRYGQRL
                     EQMYADIAATQANQLRELRRAAATQPVIDTLTQAAATILGTGSEVASDAHFTDLGGDSL
                     SALTLSNLLSDFFGFEVPVGTIVNPATNLAQLAQHIEAQRTAGDRRPSFTTVHGADATE
                     IRASELTLDKFIDAETLRAAPGLPKVTTEPRTVLLSGANGWLGRFLTLQWLERLAPVGG
                     TLITIVRGRDDAAARARLTQAYDTDPELSRRFAELADRHLRVVAGDIGDPNLGLTPEIW
                     HRLAAEVDLVVHPAALVNHVLPYRQLFGPNVVGTAEVIKLALTERIKPVTYLSTVSVAM
                     GIPDFEEDGDIRTVSPVRPLDGGYANGYGNSKWAGEVLLREAHDLCGLPVATFRSDMIL
                     AHPRYRGQVNVPDMFTRLLLSLLITGVAPRSFYIGDGERPRAHYPGLTVDFVAEAVTTL
                     GAQQREGYVSYDVMNPHDDGISLDVFVDWLIRAGHPIDRVDDYDDWVRRFETALTALPE
                     KRRAQTVLPLLHAFRAPQAPLRGAPEPTEVFHAAVRTAKVGPGDIPHLDEALIDKYIRD
                     LREFGLI"
     CDS             8722..8739
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      8806..8853
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
     5161 ggtcgcggat ccgaattcat gaccagcgat gttcacgacg ccacagacgg cgtcaccgaa
     5221 accgcactcg acgacgagca gtcgacccgc cgcatcgccg agctgtacgc caccgatccc
     5281 gagttcgccg ccgccgcacc gttgcccgcc gtggtcgacg cggcgcacaa acccgggctg
     5341 cggctggcag agatcctgca gaccctgttc accggctacg gtgaccgccc ggcgctggga
     5401 taccgcgccc gtgaactggc caccgacgag ggcgggcgca ccgtgacgcg tctgctgccg
     5461 cggttcgaca ccctcaccta cgcccaggtg tggtcgcgcg tgcaagcggt cgccgcggcc
     5521 ctgcgccaca acttcgcgca gccgatctac cccggcgacg ccgtcgcgac gatcggtttc
     5581 gcgagtcccg attacctgac gctggatctc gtatgcgcct acctgggcct cgtgagtgtt
     5641 ccgctgcagc acaacgcacc ggtcagccgg ctcgccccga tcctggccga ggtcgaaccg
     5701 cggatcctca ccgtgagcgc cgaatacctc gacctcgcag tcgaatccgt gcgggacgtc
     5761 aactcggtgt cgcagctcgt ggtgttcgac catcaccccg aggtcgacga ccaccgcgac
     5821 gcactggccc gcgcgcgtga acaactcgcc ggcaagggca tcgccgtcac caccctggac
     5881 gcgatcgccg acgagggcgc cgggctgccg gccgaaccga tctacaccgc cgaccatgat
     5941 cagcgcctcg cgatgatcct gtacacctcg ggttccaccg gcgcacccaa gggtgcgatg
     6001 tacaccgagg cgatggtggc gcggctgtgg accatgtcgt tcatcacggg tgaccccacg
     6061 ccggtcatca acgtcaactt catgccgctc aaccacctgg gcgggcgcat ccccatttcc
     6121 accgccgtgc agaacggtgg aaccagttac ttcgtaccgg aatccgacat gtccacgctg
     6181 ttcgaggatc tcgcgctggt gcgcccgacc gaactcggcc tggttccgcg cgtcgccgac
     6241 atgctctacc agcaccacct cgccaccgtc gaccgcctgg tcacgcaggg cgccgacgaa
     6301 ctgaccgccg agaagcaggc cggtgccgaa ctgcgtgagc aggtgctcgg cggacgcgtg
     6361 atcaccggat tcgtcagcac cgcaccgctg gccgcggaga tgagggcgtt cctcgacatc
     6421 accctgggcg cacacatcgt cgacggctac gggctcaccg agaccggcgc cgtgacacgc
     6481 gacggtgtga tcgtgcggcc accggtgatc gactacaagc tgatcgacgt tcccgaactc
     6541 ggctacttca gcaccgacaa gccctacccg cgtggcgaac tgctggtcag gtcgcaaacg
     6601 ctgactcccg ggtactacaa gcgccccgag gtcaccgcga gcgtcttcga ccgggacggc
     6661 tactaccaca ccggcgacgt catggccgag accgcacccg accacctggt gtacgtggac
     6721 cgtcgcaaca acgtcctcaa actcgcgcag ggcgagttcg tggcggtcgc caacctggag
     6781 gcggtgttct ccggcgcggc gctggtgcgc cagatcttcg tgtacggcaa cagcgagcgc
     6841 agtttccttc tggccgtggt ggtcccgacg ccggaggcgc tcgagcagta cgatccggcc
     6901 gcgctcaagg ccgcgctggc cgactcgctg cagcgcaccg cacgcgacgc cgaactgcaa
     6961 tcctacgagg tgccggccga tttcatcgtc gagaccgagc cgttcagcgc cgccaacggg
     7021 ctgctgtcgg gtgtcggaaa actgctgcgg cccaacctca aagaccgcta cgggcagcgc
     7081 ctggagcaga tgtacgccga tatcgcggcc acgcaggcca accagttgcg cgaactgcgg
     7141 cgcgcggccg ccacacaacc ggtgatcgac accctcaccc aggccgctgc cacgatcctc
     7201 ggcaccggga gcgaggtggc atccgacgcc cacttcaccg acctgggcgg ggattccctg
     7261 tcggcgctga cactttcgaa cctgctgagc gatttcttcg gtttcgaagt tcccgtcggc
     7321 accatcgtga acccggccac caacctcgcc caactcgccc agcacatcga ggcgcagcgc
     7381 accgcgggtg accgcaggcc gagtttcacc accgtgcacg gcgcggacgc caccgagatc
     7441 cgggcgagtg agctgaccct ggacaagttc atcgacgccg aaacgctccg ggccgcaccg
     7501 ggtctgccca aggtcaccac cgagccacgg acggtgttgc tctcgggcgc caacggctgg
     7561 ctgggccggt tcctcacgtt gcagtggctg gaacgcctgg cacctgtcgg cggcaccctc
     7621 atcacgatcg tgcggggccg cgacgacgcc gcggcccgcg cacggctgac ccaggcctac
     7681 gacaccgatc ccgagttgtc ccgccgcttc gccgagctgg ccgaccgcca cctgcgggtg
     7741 gtcgccggtg acatcggcga cccgaatctg ggcctcacac ccgagatctg gcaccggctc
     7801 gccgccgagg tcgacctggt ggtgcatccg gcagcgctgg tcaaccacgt gctcccctac
     7861 cggcagctgt tcggccccaa cgtcgtgggc acggccgagg tgatcaagct ggccctcacc
     7921 gaacggatca agcccgtcac gtacctgtcc accgtgtcgg tggccatggg gatccccgac
     7981 ttcgaggagg acggcgacat ccggaccgtg agcccggtgc gcccgctcga cggcggatac
     8041 gccaacggct acggcaacag caagtgggcc ggcgaggtgc tgctgcggga ggcccacgat
     8101 ctgtgcgggc tgcccgtggc gacgttccgc tcggacatga tcctggcgca tccgcgctac
     8161 cgcggtcagg tcaacgtgcc agacatgttc acgcgactcc tgttgagcct cttgatcacc
     8221 ggcgtcgcgc cgcggtcgtt ctacatcgga gacggtgagc gcccgcgggc gcactacccc
     8281 ggcctgacgg tcgatttcgt ggccgaggcg gtcacgacgc tcggcgcgca gcagcgcgag
     8341 ggatacgtgt cctacgacgt gatgaacccg cacgacgacg ggatctccct ggatgtgttc
     8401 gtggactggc tgatccgggc gggccatccg atcgaccggg tcgacgacta cgacgactgg
     8461 gtgcgtcggt tcgagaccgc gttgaccgcg cttcccgaga agcgccgcgc acagaccgta
     8521 ctgccgctgc tgcacgcgtt ccgcgctccg caggcaccgt tgcgcggcgc acccgaaccc
     8581 acggaggtgt tccacgccgc ggtgcgcacc gcgaaggtgg gcccgggaga catcccgcac
     8641 ctcgacgagg cgctgatcga caagtacata cgcgatctgc gtgagttcgg tctgatctga
     8701 aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
     8761 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     8821 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
//
