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Rita Galhano, Adriana Illana, Lauren S. Ryder, Julio Rodríguez-Romero, Marie Demuez, Muhammad Badaruddin, Ana Lilia Martinez-Rocha, Darren M. Soanes, David J. Studholme, Nicholas J. Talbot, Ane Sesma
Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus
www.plospathogens.org
www.plospathogens.org
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-0.0053 Tc
0 0 Td
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q
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BT
/F7 1 Tf
1 TL
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[(\r)]TJ
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1 j
1 J
0 w
7.4999 0 0 6.6659 325.2472 497.707 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 328.7622 499.4078 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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Q
q
1 j
1 J
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7.4999 0 0 6.6659 343.559 497.707 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
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Q
q
1 j
1 J
0 w
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BT
/F7 1 Tf
1 TL
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3.5149 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
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(although)Tj
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(\(conidiation,)Tj
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(and)Tj
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(appressorium)Tj
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(formation\),)Tj
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(oxidative)Tj
3.8664 0 Td
(stress)Tj
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(\(methyl)Tj
3.3278 0 Td
(viologen)Tj
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(treatment\))Tj
4.473 0 Td
(and)Tj
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(carbon)Tj
3.0104 0 Td
(starvation)Tj
4.1953 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
2.0749 0 Td
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2.4321 0 Td
(we)Tj
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(reveal)Tj
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(the)Tj
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(involvement)Tj
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(of)Tj
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(this)Tj
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(regulator)Tj
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3.2995 0 Td
(cell)Tj
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(patterning)Tj
4.3937 0 Td
(and)Tj
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(virulence)Tj
3.906 0 Td
(in)Tj
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1.0375 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F12 1 Tf
1.3209 0 Td
[()11()-20()-3()6(\n)]TJ
/F7 1 Tf
(.)Tj
2.9934 0 Td
(Among)Tj
3.2428 0 Td
(the)Tj
1.474 0 Td
(genes)Tj
2.4378 0 Td
(regulated)Tj
3.9401 0 Td
(by)Tj
-35.257 -1.3039 Td
(Tpc1)Tj
2.2451 0 Td
(we)Tj
1.3209 0 Td
(found)Tj
/F12 1 Tf
2.6192 0 Td
[()4()20()]TJ
/F7 1 Tf
(,)Tj
3.1578 0 Td
(an)Tj
1.2018 0 Td
(important)Tj
4.2803 0 Td
(component)Tj
4.8075 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4626 0 Td
(fungal)Tj
2.7269 0 Td
(NADPH)Tj
3.7021 0 Td
(complex.)Tj
3.8437 0 Td
(Significantly,)Tj
-32.3827 -1.2982 Td
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3.6453 0 Td
(with)Tj
2.0012 0 Td
(Mst12)Tj
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(and)Tj
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(mis-localises)Tj
5.2893 0 Td
(in)Tj
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(the)Tj
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1.474 0 Td
( )Tj
/F12 1 Tf
[()11()10( )]TJ
/F7 1 Tf
2.9934 0 Td
(background,)Tj
5.2554 0 Td
(linking)Tj
3.0557 0 Td
(Tpc1)Tj
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(this)Tj
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(pathogenicity-associated)Tj
10.0403 0 Td
(MAPK)Tj
3.0274 0 Td
(signalling)Tj
4.0704 0 Td
(pathway.)Tj
3.7927 0 Td
(We)Tj
1.6101 0 Td
(provide)Tj
3.2882 0 Td
(mechanistic)Tj
5.0173 0 Td
(insight)Tj
2.9536 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
-35.6425 -1.3039 Td
(role)Tj
1.7688 0 Td
(of)Tj
1.0148 0 Td
(Tpc1,)Tj
2.4718 0 Td
(a)Tj
0.652 0 Td
(key)Tj
1.5931 0 Td
(regulator)Tj
3.8664 0 Td
(of)Tj
1.0148 0 Td
(polarity)Tj
3.3164 0 Td
(in)Tj
/F12 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F12 1 Tf
1.3152 0 Td
[()11()-20()-3()6(\n)]TJ
/F7 1 Tf
2.7723 0 Td
(that)Tj
1.7802 0 Td
(controls)Tj
3.4809 0 Td
(growth,)Tj
3.2995 0 Td
(autophagy)Tj
4.388 0 Td
(and)Tj
1.7291 0 Td
(sep-)Tj
-35.5008 -1.2983 Td
(tin-mediated)Tj
5.4028 0 Td
(reorientation)Tj
5.4879 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(F-actin)Tj
3.0726 0 Td
(cytoskeleton)Tj
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(to)Tj
1.0262 0 Td
(facilitate)Tj
3.5999 0 Td
(plant)Tj
2.2677 0 Td
(colonisation.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 353.0267 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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/F6 1 Tf
0 -1.5023 Td
(M)Tj
/F0 1 Tf
(.)Tj
/F6 1 Tf
1.3275 0 Td
(oryzae)Tj
3.1843 0 Td
(TPC1)Tj
/F0 1 Tf
2.7591 0 Td
(mutants)Tj
3.7465 0 Td
(show)Tj
2.5275 0 Td
(defects)Tj
3.4111 0 Td
(in)Tj
0.9827 0 Td
(development)Tj
5.8772 0 Td
(and)Tj
-23.8159 -1.167 Td
(pathogenicity)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 304.044 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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1.1055 0 Td
(order)Tj
2.4207 0 Td
(to)Tj
1.0262 0 Td
(identify)Tj
3.2938 0 Td
(novel)Tj
2.4038 0 Td
(infection-related)Tj
6.8654 0 Td
(genes)Tj
2.4435 0 Td
(we)Tj
1.3266 0 Td
(screened)Tj
3.719 0 Td
(a)Tj
0.6577 0 Td
(total)Tj
2.0012 0 Td
(of)Tj
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(300)Tj
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(T-DNA)Tj
3.3391 0 Td
(transfor-)Tj
-33.2671 -1.3039 Td
(mants)Tj
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(for)Tj
1.3833 0 Td
(their)Tj
2.109 0 Td
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1.0261 0 Td
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2.4718 0 Td
(rice)Tj
1.7064 0 Td
(roots)Tj
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(a)Tj
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0.6577 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F12 1 Tf
1.3152 0 Td
[()11()-20()-3()6(\n)]TJ
/F7 1 Tf
2.7723 0 Td
(insertional)Tj
4.49 0 Td
(library[)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
4.7282 0 Td
(The)Tj
1.7858 0 Td
(M1422)Tj
-34.469 -1.2982 Td
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3.1464 0 Td
(developed)Tj
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(very)Tj
1.9276 0 Td
(restricted)Tj
3.9855 0 Td
(disease)Tj
3.016 0 Td
(lesions)Tj
2.9367 0 Td
(on)Tj
1.2756 0 Td
(roots)Tj
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(and)Tj
1.7291 0 Td
(was)Tj
1.7008 0 Td
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3.3449 0 Td
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1.3889 0 Td
(further)Tj
3.0274 0 Td
(charac-)Tj
-34.0098 -1.2983 Td
(terization)Tj
4.0478 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1A)Tj
0 g
(\).)Tj
1.9446 0 Td
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1.5137 0 Td
(leaves,)Tj
2.7892 0 Td
(M1422)Tj
3.0161 0 Td
(produced)Tj
4.0478 0 Td
(only)Tj
1.9729 0 Td
(a)Tj
0.6577 0 Td
(small)Tj
2.3244 0 Td
(number)Tj
3.4185 0 Td
(of)Tj
1.0148 0 Td
(resistant-type)Tj
5.6296 0 Td
(lesions)Tj
-34.1969 -1.3039 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1B)Tj
0 g
1.2756 0 Td
(and)Tj
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1.7291 0 Td
(S1A)Tj
1.8538 0 Td
(Fig)Tj
0 g
(\).)Tj
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(and)Tj
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(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(wild-type)Tj
3.9912 0 Td
(\()Tj
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(Fig)Tj
1.8198 0 Td
(1C)Tj
0 g
(\).)Tj
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1.7858 0 Td
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3.8097 0 Td
(site)Tj
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(T-DNA)Tj
3.3392 0 Td
(within)Tj
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(M1422)Tj
3.016 0 Td
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(0.9)Tj
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(downstream)Tj
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1.0148 0 Td
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(MGG_01285)Tj
5.4198 0 Td
(in)Tj
1.0319 0 Td
(the)Tj
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1.474 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
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1.3152 0 Td
[()11()-20()-3()6(\n)]TJ
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2.7723 0 Td
(genome)Tj
3.4072 0 Td
(\()Tj
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(S1B)Tj
2.092 0 Td
(Fig)Tj
0 g
(\).)Tj
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(a)Tj
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(Zn\(II\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 372.1889 198.3685 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
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BT
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1 TL
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(Cys)Tj
ET
Q
q
1 j
1 J
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7.4999 0 0 6.6659 390.5574 198.3685 cm
BT
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1 TL
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(6)Tj
ET
Q
q
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[(\r)75()20()16()-3()1()-9()10()12()7()4()11()]TJ
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[(!)9()6()-4()11()11()]TJ
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[(!)9()11()]TJ
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[()23()10()4()16()-9()14()-3()]TJ
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[()-2()11()22()7()20()10()]TJ
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[()]TJ
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(\()Tj
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[(\r)]TJ
/F7 1 Tf
(\).)Tj
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1.7802 0 Td
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(http://fungi.ensembl.org/inde)Tj
12.1831 0 Td
(x.html)Tj
0 g
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3.0274 0 Td
(MG8\).)Tj
2.8686 0 Td
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1.7802 0 Td
[(\r)]TJ
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(a)Tj
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(putative)Tj
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(nuclear)Tj
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(localisation)Tj
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(\(NLS\))Tj
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(and)Tj
1.7291 0 Td
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(Zn\(II\))Tj
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Q
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1 J
0 w
7.4999 0 0 6.6659 225.0708 146.3244 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
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1 J
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BT
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1 TL
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Q
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1 J
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7.4999 0 0 6.6659 243.4393 146.3244 cm
BT
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1 TL
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(6)Tj
ET
Q
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(S1B)Tj
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(Fig)Tj
0 g
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(hygromycin)Tj
5.0626 0 Td
(phosphotransferase)Tj
7.9993 0 Td
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(a)Tj
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(probe)Tj
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(\()Tj
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(S1C)Tj
2.177 0 Td
(Fig)Tj
0 g
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(S2A)Tj
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(and)Tj
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(S2B)Tj
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(M1422)Tj
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