Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux

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Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux

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dc.contributor.author Stabler, Richard A. en_GB
dc.contributor.author Dawson, Lisa F. en_GB
dc.contributor.author Oyston, Petra C. F. en_GB
dc.contributor.author Titball, Richard W. en_GB
dc.contributor.author Wade, Jim en_GB
dc.contributor.author Hinds, Jason en_GB
dc.contributor.author Witney, Adam A. en_GB
dc.contributor.author Wren, Brendan W. en_GB
dc.date.accessioned 2010-06-10T15:03:00Z en_GB
dc.date.accessioned 2011-01-25T11:47:08Z en_US
dc.date.accessioned 2013-03-20T14:49:22Z
dc.date.issued 2008 en_GB
dc.description.abstract Background: Human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. Results: The microarray consists of 4958 reporters from 151 bacterial species and include genes for the identification of individual bacterial species as well as mobile genetic elements (transposons, plasmid and phage), virulence genes and antibiotic resistance genes. The ASP microarray was validated with nineteen bacterial pathogens species, including Francisella tularensis, Clostridium difficile, Staphylococcus aureus, Enterococcus faecium and Stenotrophomonas maltophilia. The ASP microarray identified these bacteria, and provided information on potential antibiotic resistance (eg sufamethoxazole resistance and sulfonamide resistance) and virulence determinants including genes likely to be acquired by horizontal gene transfer (e.g. an alpha-haemolysin). Conclusion: The ASP microarray has potential in the clinic as a diagnostic tool, as a research tool for both known and emerging pathogens, and as an early warning system for pathogenic bacteria that have been recently modified either naturally or deliberately. en_GB
dc.identifier.citation 8, article 177 en_GB
dc.identifier.doi 10.1186/1471-2180-8-177 en_GB
dc.identifier.uri http://hdl.handle.net/10036/104626 en_GB
dc.language.iso en en_GB
dc.publisher BioMed Central en_GB
dc.relation.url http://www.biomedcentral.com/1471-2180/8/177 en_GB
dc.relation.url http://dx.doi.org/10.1186/1471-2180-8-177 en_GB
dc.title Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux en_GB
dc.type Article en_GB
dc.date.available 2010-06-10T15:03:00Z en_GB
dc.date.available 2011-01-25T11:47:08Z en_US
dc.date.available 2013-03-20T14:49:22Z
dc.identifier.issn 1471-2180 en_GB
dc.description © 2008 Stabler et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_GB
dc.identifier.journal BMC Microbiology en_GB


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