Now showing items 1-20 of 31

  • Analysis of known bacterial protein vaccine antigens reveals biased physical properties and amino acid composition 

    Mayers, Carl; Duffield, Melanie; Rowe, Sonya; Miller, Julie; Lingard, Bryan; Hayward, Sarah; Titball, Richard W. (Hindawi Publishing Corporation, 2003)
    Many vaccines have been developed from live attenuated forms of bacterial pathogens or from killed bacterial cells. However, an increased awareness of the potential for transient side-effects following vaccination has ...
  • ATP-binding cassette systems in Burkholderia pseudomallei and Burkholderia mallei 

    Harland, David N.; Dassa, Elie; Titball, Richard W.; Brown, Katherine A.; Atkins, Helen S. (BioMed Central, 2007)
    Background: ATP binding cassette (ABC) systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and ...
  • A bacterial process for selenium nanosphere assembly 

    Debieux, Charles M.; Dridge, Elizabeth J.; Mueller, Claudia M; Splatt, Peter; Paszkiewicz, K; Knight, Iona; Florance, Hannah; Love, John; Titball, Richard W.; Lewis, Richard J; Richardson, David J; Butler, Clive S (2011-08-16)
    During selenate respiration by Thauera selenatis, the reduction of selenate results in the formation of intracellular selenium (Se) deposits that are ultimately secreted as Se nanospheres of approximately 150 nm in diameter. ...
  • A Burkholderia pseudomallei macrophage infectivity potentiator-like protein has rapamycin-inhibitable peptidylprolyl isomerase activity and pleiotropic effects on virulence 

    Norville, IH; Harmer, Nicholas; Harding, SV; Fischer, Gunter; Keith, KE; Brown, Katherine A.; Sarkar-Tyson, M; Titball, Richard W. (American Society for Microbiology, 2011-11)
    Macrophage infectivity potentiators (Mips) are a group of virulence factors encoded by pathogenic bacteria such as Legionella, Chlamydia, and Neisseria species. Mips are part of the FK506-binding protein (FKBP) family, ...
  • Burkholderia pseudomallei sequencing identifies genomic clades with distinct recombination, accessory, and epigenetic profiles 

    Nandi, T; Holden, Matthew; Didelot, X; Mehershahi, K; Boddey, J; Beacham, I; Peak, I; Harting, J; Baybayan, P; Guo, Y; Wang, S; How, LC; Sim, B; Essex-Lopresti, AE; Sarkar-Tyson, M; Nelson, M; Smither, S; Ong, C; Aw, LT; Hoon, CH; Michell, S; Studholme, David J.; Titball, Richard W.; Chen, SL; Parkhill, J; Tan, P (Cold Spring Harbor Laboratory Press, 2014-09-18)
    Burkholderia pseudomallei (Bp) is the causative agent of the infectious disease melioidosis. To investigate population diversity, recombination, and horizontal gene transfer in closely related Bp isolates, we performed ...
  • Clostridium perfringens epsilon toxin H149A mutant as a platform for receptor binding studies 

    Bokori-Brown, Monika; Kokkinidou, Maria C.; Savva, Christos G.; Fernandes da Costa, Sérgio P.; Naylor, Claire E.; Cole, Ambrose R.; Moss, David S.; Basak, Ajit K.; Titball, Richard W. (Wiley, 2013-05)
    Clostridium perfringens epsilon toxin (Etx) is a pore-forming toxin responsible for a severe and rapidly fatal enterotoxemia of ruminants. The toxin is classified as a category B bioterrorism agent by the U.S. Government ...
  • Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia 

    Bokori-Brown, Monika; Hall, CA; Vance, C; Fernandes da Costa, Sérgio P.; Savva, CG; Naylor, CE; Cole, Ambrose R.; Basak, Ajit K.; Moss, DS; Titball, Richard W. (Elsevier, 2014-05-13)
    Epsilon toxin (Etx) is a β-pore-forming toxin produced by Clostridium perfringens toxinotypes B and D and plays a key role in the pathogenesis of enterotoxemia, a severe, often fatal disease of ruminants that causes ...
  • Comparative genomic characterization of Francisella tularensis strains belonging to low and high virulence subspecies 

    Champion, Mia D.; Zeng, Qiandong; Nix, Eli B.; Nano, Francis E.; Keim, Paul; Kodira, Chinnappa D.; Borowsky, Mark; Young, Sarah; Koehrsen, Michael; Engels, Reinhard; Pearson, Matthew; Howarth, Clint; Larson, Lisa; White, Jared; Alvarado, Lucia; Forsman, Mats; Bearden, Scott W.; Sjöstedt, Anders; Titball, Richard W.; Michell, S; Birren, Bruce; Galagan, James (Public Library of Science (PLoS), 2009)
    Tularemia is a geographically widespread, severely debilitating, and occasionally lethal disease in humans. It is caused by infection by a gram-negative bacterium, Francisella tularensis. In order to better understand its ...
  • Conjugation of Y. pestis F1-antigen to gold nanoparticles improves immunogenicity 

    Gregory, Anthony; Williamson, E. Diane; Prior, JL; Butcher, WA; Thompson, IJ; Shaw, AM; Titball, Richard W. (Elsevier, 2012-11-06)
    The efficacy of 15 nm gold nanoparticles (AuNP) coated with Yersinia pestis F1-antigen, as an immunogen in mice, has been assessed. The nanoparticles were decorated with F1-antigen using N-hydroxysuccinimide and ...
  • Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux 

    Stabler, Richard A.; Dawson, Lisa F.; Oyston, Petra C. F.; Titball, Richard W.; Wade, Jim; Hinds, Jason; Witney, Adam A.; Wren, Brendan W. (BioMed Central, 2008)
    Background: Human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. We describe the construction ...
  • Differences in carbon source utilisation distinguish Campylobacter jejuni from Campylobacter coli 

    Wagley, S; Newcombe, J; Laing, E; Yusuf, E; Sambles, CM; Studholme, David J.; La Ragione, RM; Titball, Richard W.; Champion, OL (BioMed Central, 2014-10-28)
    BACKGROUND: Campylobacter jejuni and C. coli are human intestinal pathogens that are the most frequent causes of bacterial foodborne gastroenteritis in humans in the UK. In this study, we aimed to characterise the metabolic ...
  • DNA vaccines: improving expression of antigens 

    Garmory, Helen S.; Brown, Katherine A.; Titball, Richard W. (BioMed Central, 2003)
    DNA vaccination is a relatively recent development in vaccine methodology. It is now possible to undertake a rational step-by-step approach to DNA vaccine design. Strategies may include the incorporation of immunostimulatory ...
  • Draft Genome Sequence of the Enteropathogenic Bacterium Campylobacter jejuni Strain cj255. 

    Siddiqui, FM; Ibrahim, M; Noureen, N; Noreen, Z; Titball, Richard W.; Champion, OL; Wren, BW; Studholme, David J.; Bokhari, H (American Society for Microbiology: Genome Announcements, 2015-10-22)
    The enteropathogen Campylobacter jejuni is a global health disaster, being one of the leading causes of bacterial gastroenteritis. Here, we present the draft genome sequence of C. jejuni strain cj255, isolated from a chicken ...
  • Functional Analysis of the Role of Toxin-Antitoxin (TA) Loci in Bacterial Persistence. 

    Butt, Aaron T.; Titball, Richard W. (Springer New York, 2016)
    We have developed a method to analyze the functionality of putative TA loci by expressing them in Escherichia coli. Here, we describe the procedure for cloning recombinant TA genes into inducible plasmids and expressing ...
  • Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4 

    Chaudhuri, Roy R.; Ren, Chuan-Peng; Desmond, Leah; Vincent, Gemma A.; Silman, Nigel J.; Brehm, John K.; Elmore, Michael J.; Hudson, Michael J.; Forsman, Mats; Isherwood, Karen E.; Guryčová, Darina; Minton, Nigel P.; Titball, Richard W.; Pallen, Mark J.; Vipond, Richard (Public Library of Science, 2007)
    Background: Francisella tularensis causes tularaemia, a life-threatening zoonosis, and has potential as a biowarfare agent. F. tularensis subsp. tularensis, which causes the most severe form of tularaemia, is usually ...
  • Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243. 

    Dowling, AJ; Wilkinson, PA; Holden, Matthew; Quail, MA; Bentley, SD; Reger, J; Waterfield, NR; Titball, Richard W.; ffrench-Constant, Richard (Public Library of Science, 2010-12-22)
    Burkholderia pseudomallei is an important human pathogen whose infection biology is still poorly understood. The bacterium is endemic to tropical regions, including South East Asia and Northern Australia, where it causes ...
  • A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence. 

    Nandi, T; Ong, C; Singh, AP; Boddey, J; Atkins, T; Sarkar-Tyson, M; Essex-Lopresti, AE; Chua, HH; Pearson, T; Kreisberg, JF; Nilsson, C; Ariyaratne, P; Ronning, C; Losada, L; Ruan, Y; Sung, WK; Woods, D; Titball, Richard W.; Beacham, I; Peak, I; Keim, P; Nierman, WC; Tan, P (Public Library of Science, 2010-04)
    Certain environmental microorganisms can cause severe human infections, even in the absence of an obvious requirement for transition through an animal host for replication ("accidental virulence"). To understand this ...
  • The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation 

    Butt, Aaron T.; Higman, VA; Williams, C; Crump, MP; Hemsley, CM; Harmer, Nicholas; Titball, Richard W. (Portland Press, 2014-04-15)
    TA (toxin-antitoxin) systems are widely distributed amongst bacteria and are associated with the formation of antibiotic tolerant (persister) cells that may have involvement in chronic and recurrent disease. We show that ...
  • Identification of a key residue for oligomerisation and pore-formation of Clostridium perfringens NetB. 

    Fernandes da Costa, Sérgio P.; Savva, Christos G.; Bokori-Brown, Monika; Naylor, Claire E.; Moss, David S.; Basak, Ajit K.; Titball, Richard W. (MDPI AG, 2014-03-12)
    Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant ...
  • Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei 

    Butt, Aaron Trevor; Müller, C; Harmer, Nicholas; Titball, Richard W. (Wiley:, 2013-01)
    Type II toxin-antitoxin (TA) systems are believed to be widely distributed amongst bacteria although their biological functions are not clear. We have identified eight candidate TA systems in the genome of the human pathogen ...