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dc.contributor.authorWondimu, ET
dc.contributor.authorZhang, Q
dc.contributor.authorJin, Z
dc.contributor.authorFu, M
dc.contributor.authorTorregrossa, R
dc.contributor.authorWhiteman, M
dc.contributor.authorYang, G
dc.contributor.authorWu, L
dc.contributor.authorWang, R
dc.date.accessioned2021-08-06T12:11:19Z
dc.date.issued2021-08-04
dc.description.abstractHydrogen sulfide (H2 S) is a gasotransmitter that regulates both physiological and pathophysiological processes in mammalian cells. Recent studies have demonstrated that H2 S promotes aerobic energy production in the mitochondria in response to hypoxia, but its effect on anaerobic energy production has yet to be established. Glycolysis is the anaerobic process by which ATP is produced through the metabolism of glucose. Mammalian red blood cells (RBCs) extrude mitochondria and nucleus during erythropoiesis. These cells would serve as a unique model to observe the effect of H2 S on glycolysis-mediated energy production. The purpose of this study was to determine the effect of H2 S on glycolysis-mediated energy production in mitochondria-free mouse RBCs. Western blot analysis showed that the only H2 S-generating enzyme expressed in mouse RBCs is 3-mercaptopyruvate sulfurtransferase (MST). Supplement of the substrate for MST stimulated, but the inhibition of the same suppressed, the endogenous production of H2 S. Both exogenously administered H2 S salt and MST-derived endogenous H2 S stimulated glycolysis-mediated ATP production. The effect of NaHS on ATP levels was not affected by oxygenation status. On the contrary, hypoxia increased intracellular H2 S levels and MST activity in mouse RBCs. The mitochondria-targeted H2 S donor, AP39, did not affect ATP levels of mouse RBCs. NaHS at low concentrations (3-100 μM) increased ATP levels and decreased cell viability after 3 days of incubation in vitro. Higher NaHS concentrations (300-1000 μM) lowered ATP levels, but prolonged cell viability. H2 S may offer a cytoprotective effect in mammalian RBCs to maintain oxygen-independent energy production.en_GB
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC)en_GB
dc.description.sponsorshipHeart and Stroke Foundation of Ontarioen_GB
dc.identifier.citationPublished online 4 August 2021en_GB
dc.identifier.doi10.1002/jcp.30544
dc.identifier.grantnumberRGPIN-2017-04392en_GB
dc.identifier.urihttp://hdl.handle.net/10871/126691
dc.language.isoenen_GB
dc.publisherWileyen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/34346059en_GB
dc.rights.embargoreasonUnder embargo until 4 August 2022 in compliance with publisher policyen_GB
dc.rights© 2021 Wileyen_GB
dc.subject3-mercaptopyruvate sulfur transferaseen_GB
dc.subjectATP productionen_GB
dc.subjectglycolysisen_GB
dc.subjecthypoxiaen_GB
dc.subjectred blood cellen_GB
dc.titleEffect of hydrogen sulfide on glycolysis-based energy production in mouse erythrocytesen_GB
dc.typeArticleen_GB
dc.date.available2021-08-06T12:11:19Z
exeter.place-of-publicationUnited Statesen_GB
dc.descriptionThis is the author accepted manuscript. The final version is available from Wiley via the DOI in this recorden_GB
dc.identifier.eissn1097-4652
dc.identifier.journalJournal of Cellular Physiologyen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2021-07-24
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2021-08-04
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2021-08-06T12:07:52Z
refterms.versionFCDAM
refterms.panelAen_GB


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