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dc.contributor.authorThornton, CR
dc.contributor.authorSlaughter, DC
dc.contributor.authorDavis, Richard M.
dc.date.accessioned2013-11-20T10:00:54Z
dc.date.issued2010-10
dc.description.abstractGeotrichum candidum is a common soil-borne fungus that causes sour-rot of tomatoes, citrus fruits and vegetables, and is a major contaminant on tomato processing equipment. The aim of this work was to produce a monoclonal antibody and diagnostic assay for its detection in tomato fruit and juice. Using hybridoma technology, a cell line (FE10) was generated that produced a monoclonal antibody belonging to the immunoglobulin class M (IgM) that was specific to G. candidum and the closely related teleomorphic species Galactomyces geotrichum and anamorphic species Geotrichum europaeum and Geotrichum pseudocandidum in the G. geotrichum/G. candidum complex. The MAb did not cross-react with a wide range of unrelated fungi, including some likely to be encountered during crop production and processing. The MAb binds to an immunodominant high molecular mass (> 200 kDa) extracellular polysaccharide antigen that is present on the surface of arthroconidia and hyphae of G. candidum. The MAb was used in a highly specific enzyme-linked immunosorbent assay (ELISA) to accurately detect the fungus in infected tomato fruit and juice. Specificity of the ELISA was confirmed by sequencing of the internally transcribed spacer (ITS) 1-5.8S-ITS2 rRNA-encoding regions of fungi isolated from naturally-infected tomatoes.en_GB
dc.identifier.citationInternational Journal of Food Microbiology, 2010, Vol. 143, Issue 3, pp. 166 - 172en_GB
dc.identifier.doi10.1016/j.ijfoodmicro.2010.08.012
dc.identifier.urihttp://hdl.handle.net/10871/13974
dc.language.isoenen_GB
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0168160510004721en_GB
dc.subjectGeotrichum candidumen_GB
dc.subjectHybridomaen_GB
dc.subjectMonoclonal antibodyen_GB
dc.subjectEnzyme-linked immunosorbent assayen_GB
dc.titleDetection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISAen_GB
dc.typeArticleen_GB
dc.date.available2013-11-20T10:00:54Z
dc.identifier.issn0168-1605
dc.descriptionCopyright © 2010 Elsevier. NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Food Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Food Microbiology, 2010, Vol. 143, Issue 3, pp. 166 – 172 DOI: 10.1016/j.ijfoodmicro.2010.08.012en_GB
dc.identifier.journalInternational Journal of Food Microbiologyen_GB


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