Show simple item record

dc.contributor.authorWise, Daniel
dc.date.accessioned2014-11-19T10:02:00Z
dc.date.issued2014-06-12
dc.description.abstractPhytohormone signalling has been shown to have crucial and broad roles in plant development and survival. One of these many roles is plant immunity and response to pathogen invasion. Three main hormones salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA), have been shown to have a dramatic impact on plant immunity and determining susceptibility or resistance to pathogen attack. Post invasion defence responses are primarily regulated by a SA dependent pathway. SA mediated defence is shown to be compromised by antagonistic action of the JA and ABA signalling pathways. The multifaceted crosstalk between these three hormone signalling pathways dictates plant susceptibility and resistance. In this study we focus on the regulation of the JA signalling pathway, in particular the role of the transcription factor inhibitor, jasmonate-ZIM-domain proteins. An active form of JA, jasmonoyl–isoleucine (JA–Ile) facilitates the interaction between COI1, an F-box protein and various JAZ proteins to form SCF E3 ubiquitin ligase complexes. SCFCOI1 then tags the captive JAZ proteins for ubiquitination by the 26S proteasome, this allows for transcription of early JA-related genes. We challenged A. thaliana strains, with specific JAZ protein insertional mutations, with the model pathogen P. syringae DC3000. From this work we show a role for JAZ proteins in host susceptibility and resistance. In particular we show a decreased ability in P. syringae to proliferate in A. thaliana jaz 7 knockout mutants, as well increased susceptibility to jaz 5/10 deficient mutants. We also identify a positive relationship between coronatine associated gene expression and jaz 5/10 deficient mutants.en_GB
dc.identifier.urihttp://hdl.handle.net/10871/15895
dc.language.isoenen_GB
dc.publisherUniversity of Exeteren_GB
dc.titleThe role of Jasmonate-ZIM-Domain proteins in Systemic Immunityen_GB
dc.typeThesis or dissertationen_GB
dc.date.available2014-11-19T10:02:00Z
dc.contributor.advisorNicholas, Smirnoff
dc.publisher.departmentLife and Environmental Sciencesen_GB
dc.type.degreetitleMbyRes in Biosciencesen_GB
dc.type.qualificationlevelMasters Degreeen_GB
dc.type.qualificationnameMbyResen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record