Show simple item record

dc.contributor.authorRyder, LS
dc.contributor.authorDagdas, Yasin Fatih
dc.contributor.authorMentlak, TA
dc.contributor.authorKershaw, MJ
dc.contributor.authorThornton, CR
dc.contributor.authorSchuster, M
dc.contributor.authorChen, J
dc.contributor.authorWang, Z
dc.contributor.authorTalbot, Nicholas J.
dc.date.accessioned2014-11-19T10:15:44Z
dc.date.issued2013-02-19
dc.description.abstractThe rice blast fungus Magnaporthe oryzae infects plants with a specialized cell called an appressorium, which uses turgor to drive a rigid penetration peg through the rice leaf cuticle. Here, we show that NADPH oxidases (Nox) are necessary for septin-mediated reorientation of the F-actin cytoskeleton to facilitate cuticle rupture and plant cell invasion. We report that the Nox2-NoxR complex spatially organizes a heteroligomeric septin ring at the appressorium pore, required for assembly of a toroidal F-actin network at the point of penetration peg emergence. Maintenance of the cortical F-actin network during plant infection independently requires Nox1, a second NADPH oxidase, which is necessary for penetration hypha elongation. Organization of F-actin in appressoria is disrupted by application of antioxidants, whereas latrunculin-mediated depolymerization of appressorial F-actin is competitively inhibited by reactive oxygen species, providing evidence that regulated synthesis of reactive oxygen species by fungal NADPH oxidases directly controls septin and F-actin dynamics.en_GB
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC)en_GB
dc.description.sponsorshipNational Natural Science Foundation of Chinaen_GB
dc.description.sponsorshipHalpin Scholarshipen_GB
dc.description.sponsorshipEuropean Research Council Advanced Investigator Awarden_GB
dc.identifier.citationVol. 110, Issue 8, pp. 3179 - 3184en_GB
dc.identifier.doi10.1073/pnas.1217470110
dc.identifier.grantnumberBB/G013896/en_GB
dc.identifier.grantnumber31030004en_GB
dc.identifier.other1217470110
dc.identifier.urihttp://hdl.handle.net/10871/15896
dc.publisherPNASen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23382235en_GB
dc.rightsPlease cite published version which is available from the DOI link above.en_GB
dc.subjectCytoskeletonen_GB
dc.subjectMagnaportheen_GB
dc.subjectMicroscopy, Fluorescenceen_GB
dc.subjectNADPH Oxidaseen_GB
dc.subjectOryza sativaen_GB
dc.subjectReactive Oxygen Speciesen_GB
dc.subjectSeptinsen_GB
dc.titleNADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungusen_GB
dc.typeArticleen_GB
dc.date.available2014-11-19T10:15:44Z
exeter.place-of-publicationUnited States
dc.descriptionnotes: PMCID: PMC3581893en_GB
dc.descriptiontypes: Journal Article; Research Support, Non-U.S. Gov'ten_GB
dc.identifier.journalProc Natl Acad Sci U S Aen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record