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dc.contributor.authorMansfield, Jessica
dc.contributor.authorLittlejohn, George
dc.contributor.authorSeymour, Mark P.
dc.contributor.authorLind, Rob
dc.contributor.authorPerfect, Sarah
dc.contributor.authorMoger, Julian
dc.date.accessioned2015-11-09T12:04:18Z
dc.date.issued2013-05-21
dc.description.abstractThe growing world population puts ever-increasing demands on the agricultural and agrochemical industries to increase agricultural yields. This can only be achieved by investing in fundamental plant and agrochemical research and in the development of improved analytical tools to support research in these areas. There is currently a lack of analytical tools that provide noninvasive structural and chemical analysis of plant tissues at the cellular scale. Imaging techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy provide label-free chemically specific image contrast based on vibrational spectroscopy. Over the past decade, these techniques have been shown to offer clear advantages for a vast range of biomedical research applications. The intrinsic vibrational contrast provides label-free quantitative functional analysis, it does not suffer from photobleaching, and it allows near real-time imaging in 3D with submicrometer spatial resolution. However, due to the susceptibility of current detection schemes to optical absorption and fluorescence from pigments (such as chlorophyll), the plant science and agrochemical research communities have not been able to benefit from these techniques and their application in plant research has remained virtually unexplored. In this paper, we explore the effect of chlorophyll fluorescence and absorption in CARS and SRS microscopy. We show that with the latter it is possible to use phase-sensitive detection to separate the vibrational signal from the (electronic) absorption processes. Finally, we demonstrate the potential of SRS for a range of in planta applications by presenting in situ chemical analysis of plant cell wall components, epicuticular waxes, and the deposition of agrochemical formulations onto the leaf surface.en_GB
dc.identifier.citationVol. 85 (10), pp 5055–5063en_GB
dc.identifier.doi10.1021/ac400266a
dc.identifier.urihttp://hdl.handle.net/10871/18600
dc.language.isoenen_GB
dc.publisherAmerican Chemical Societyen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23581493en_GB
dc.subjectAgrochemicalsen_GB
dc.subjectCell Wallen_GB
dc.subjectGossypiumen_GB
dc.subjectMicroscopyen_GB
dc.subjectMolecular Imagingen_GB
dc.subjectPlant Leavesen_GB
dc.subjectSpectrum Analysis, Ramanen_GB
dc.subjectVibrationen_GB
dc.subjectWaxesen_GB
dc.subjectZea maysen_GB
dc.titleLabel-free chemically specific imaging in planta with stimulated Raman scattering microscopy.en_GB
dc.typeArticleen_GB
dc.date.available2015-11-09T12:04:18Z
dc.identifier.issn0003-2700
exeter.place-of-publicationUnited States
dc.descriptionAccepted version of article published in Analytical Chemistry, 2013, 85 (10), pp 5055–5063. DOI: 10.1021/ac400266a. Copyright © 2013 American Chemical Societyen_GB
dc.identifier.eissn1520-6882
dc.identifier.journalAnalytical Chemistryen_GB


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