Functional Analysis of the Role of Toxin-Antitoxin (TA) Loci in Bacterial Persistence.
Butt, Aaron T.
Titball, Richard W.
Methods in Molecular Biology
Springer New York
Reason for embargo
We have developed a method to analyze the functionality of putative TA loci by expressing them in Escherichia coli. Here, we describe the procedure for cloning recombinant TA genes into inducible plasmids and expressing these in E. coli. Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in E. coli and whether expression can affect persister cell formation.
Bacterial Persistence: Methods and Protocols
The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-2854-5_11
Vol. 1333, pp. 121 - 129
Place of publication