dc.contributor.author | Monteil, CL | |
dc.contributor.author | Yahara, K | |
dc.contributor.author | Studholme, DJ | |
dc.contributor.author | Mageiros, L | |
dc.contributor.author | Méric, G | |
dc.contributor.author | Swingle, B | |
dc.contributor.author | Morris, CE | |
dc.contributor.author | Vinatzer, BA | |
dc.contributor.author | Sheppard, SK | |
dc.date.accessioned | 2016-10-19T09:14:42Z | |
dc.date.issued | 2016-09-20 | |
dc.description.abstract | Many bacterial pathogens are well characterized but, in some cases, relatively little is
known about the populations from which they emerged. This limits understanding of
the molecular mechanisms underlying disease. The crop pathogen Pseudomonas
syringae sensu lato has been widely isolated from the environment, including wild
plants and components of the water cycle, and causes disease in several economically
important crops. Here, we compared genome sequences of 45 P. syringae crop
pathogen outbreak strains with 69 closely related environmental isolates. Phylogenetic
reconstruction revealed that crop pathogens emerged many times independently from
environmental populations. Unexpectedly, differences in gene content between
environmental populations and outbreak strains were minimal with most virulence
genes present in both. However, a genome-wide association study identified a small
number of genes, including the type III effector genes hopQ1 and hopD1, to be
associated with crop pathogens, but not with environmental populations, suggesting
that this small group of genes may play an important role in crop disease emergence.
Intriguingly, genome-wide analysis of homologous recombination revealed that the
locus Psyr 0346, predicted to encode a protein that confers antibiotic resistance, has
been frequently exchanged among lineages and thus may contribute to pathogen
fitness. Finally, we found that isolates from diseased crops and from components of the
water cycle, collected during the same crop disease epidemic, form a single
population. This provides the strongest evidence yet that precipitation and irrigation
water are an overlooked inoculum source for disease epidemics caused by P.
syringae. | en_GB |
dc.description.sponsorship | Caroline L. Monteil
received support from INRA and the European Union, in the framework of the Marie-Curie FP7
COFUND People Programme, through the award of an AgreenSkills’ fellowship (under grant
agreement n° 267196). Research in Boris A. Vinatzer’s laboratory and genome sequencing was
funded by the National Science Foundation of the USA (grants IOS-1354215 and DEB-1241068).
Funding for work in the Vinatzer laboratory was also provided in part by the Virginia Agricultural
Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S.
Department of Agriculture. Work carried out in the Sheppard laboratory was supported by the
Medical Research Council (MRC) grant MR/L015080/1, and the Wellcome Trust grant
088786/C/09/Z. GM was supported by a NISCHR Health Research Fellowship (HF-14-13). | en_GB |
dc.identifier.citation | Published Ahead of Print: 20 September, 2016 Microbial Genomics doi: 10.1099/mgen.0.000089 | en_GB |
dc.identifier.doi | 10.1099/mgen.0.000089 | |
dc.identifier.uri | http://hdl.handle.net/10871/23965 | |
dc.language.iso | en | en_GB |
dc.publisher | Microbiology Society | en_GB |
dc.rights | This is an open access article published by the Microbiology Society under the Creative Commons Attribution-NonCommercial License | en_GB |
dc.title | Population-genomic insights into emergence, crop-adaptation, and dissemination of Pseudomonas syringae pathogens | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-10-19T09:14:42Z | |
dc.identifier.issn | 2057-5858 | |
dc.description | This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Microbial Genomics | en_GB |