Generation of temperature sensitive mutants for the genetic study of the fission yeast spindle pole body
Methods in Molecular Biology
Humana Press (Springer Imprint)
Reason for embargo
This is the author accepted manuscript. It is currently under an indefinite embargo pending publication by the publisher. 12 month embargo to be applied on publication
The spindle pole body (SPB) in fungi is the equivalent of the animal centrosome. A number of previous studies have identified many, if not all, components of the SPB. The SPB is the structural platform for microtubule nucleation and plays important roles both in mitosis and meiosis. The SPB is absolutely essential for cell survival and its abnormalities give rise to aberrant cell division and morphogenesis. Therefore, it is crucial to understand how the SPB organises itself and how the functions of individual SPB components are regulated. We report here a procedure to generate temperature sensitive mutants in the fission yeast, Schizosaccharomyces pombe. The approach has proved useful to characterise functions of the SPB components. This original genetic manipulation is however not restricted to analysis of SPB functions, and can be suited to investigate other cellular processes in S. pombe.
This work was supported by Cancer Research UK and the Francis Crick Institute. We are grateful to Andy Early for critical reading of the manuscript.
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