Show simple item record

dc.contributor.authorBates, S
dc.date.accessioned2018-06-13T09:16:50Z
dc.date.issued2018-06-11
dc.description.abstractCandida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. albicans have raised questions on its role in this organism, with the components analysed to date demonstrating differing levels of importance in the processes of mitotic exit, cytokinesis and cell separation. This work focuses on the role of the Cdc15 kinase in C. albicans and demonstrates that, similar to Saccharomyces cerevisiae, it plays an essential role in signalling for mitotic exit and cytokinesis. Cells depleted of Cdc15 developed into elongated filaments, a common response to cell cycle arrest in C. albicans. These filaments emerged exclusively from large budded cells, contained two nuclear bodies and exhibited a hyper-extended spindle, all characteristic of these cells failing to exit mitosis. Furthermore these filaments displayed a clear cytokinesis defect, and CDC15 over-expression led to aberrant cell separation following hyphal morphogenesis. Together, these results are consistent with Cdc15 playing an essential role in signalling for mitotic exit, cytokinesis and cell separation in C. albicans.en_GB
dc.description.sponsorshipThis work was supported by a UK Biotechnology and Biological Sciences Research Council (BBSRC) project grant (No. BB/F009232/1) to S.B.en_GB
dc.identifier.citationVol. 8, pp. 8899 -en_GB
dc.identifier.doi10.1038/s41598-018-27157-y
dc.identifier.other10.1038/s41598-018-27157-y
dc.identifier.urihttp://hdl.handle.net/10871/33186
dc.language.isoenen_GB
dc.publisherNature Publishing Groupen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/29891974en_GB
dc.rights© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_GB
dc.titleCandida albicans Cdc15 is essential for mitotic exit and cytokinesis.en_GB
dc.typeArticleen_GB
dc.date.available2018-06-13T09:16:50Z
dc.identifier.issn2045-2322
exeter.place-of-publicationEnglanden_GB
dc.descriptionThis is the author accepted manuscript. The final version is available from Nature Publishing Group via the DOI in this record.en_GB
dc.identifier.journalScientific Reportsen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record