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dc.contributor.authorTaylor, M
dc.contributor.authorMurray, J
dc.contributor.authorPrice, C
dc.contributor.authorAves, Stephen
dc.contributor.authorMoore, Karen
dc.date.accessioned2013-04-30T14:00:59Z
dc.date.issued2011-11-10
dc.description.abstractInitiation of DNA replication in eukaryotes is a highly conserved and ordered process involving the co-ordinated, stepwise association of distinct proteins at multiple origins of replication throughout the genome. Here, taking Schizosaccharomyces pombe as a model, the role of Rad4(TopBP1) in the assembly of the replication complex has been examined. Quantitative chromatin immunoprecipitation experiments confirm that Rad4(TopBP1) associates with origins of DNA replication and, in addition, demonstrate that the protein is not present within the active replisome. A direct interaction between Rad4(TopBP1) and Mcm10 is shown and this is reflected in the Rad4(TopBP1)-dependent origin association of Mcm10. Rad4(TopBP1) is also shown to interact with Sld2 and Sld3 and to be required for the stable origin association of these two proteins. Rad4(TopBP1) chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4(TopBP1) origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4(TopBP1) at these distinct sites.en_GB
dc.identifier.citationVol. 10, Issue 11, pp. 1154 - 1163en_GB
dc.identifier.doi10.1016/j.dnarep.2011.09.001
dc.identifier.urihttp://hdl.handle.net/10871/8721
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1568786411002369en_GB
dc.subjectCell Cycle Checkpointsen_GB
dc.subjectCell Cycle Proteinsen_GB
dc.subjectDNA Replicationen_GB
dc.subjectDNA-Binding Proteinsen_GB
dc.subjectProtein Bindingen_GB
dc.subjectReplication Originen_GB
dc.subjectSchizosaccharomycesen_GB
dc.subjectSchizosaccharomyces pombe Proteinsen_GB
dc.subjectTransglutaminasesen_GB
dc.titleMcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1).en_GB
dc.typeArticleen_GB
dc.date.available2013-04-30T14:00:59Z
exeter.place-of-publicationNetherlands
dc.description© Elsevier, 2011. This is a post-print version of the article. The definitive version is available from: http://www.sciencedirect.com/science/article/pii/S1568786411002369. Deposited in accordance with SHERPA RoMEO guidelinesen_GB
dc.identifier.journalDNA Repairen_GB


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