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dc.contributor.authorMurphy, TM
dc.contributor.authorWong, CC
dc.contributor.authorArseneault, L
dc.contributor.authorBurrage, J
dc.contributor.authorMacdonald, Ruby
dc.contributor.authorHannon, E
dc.contributor.authorFisher, HL
dc.contributor.authorAmbler, A
dc.contributor.authorMoffitt, TE
dc.contributor.authorCaspi, A
dc.contributor.authorMill, J
dc.date.accessioned2016-01-05T12:42:44Z
dc.date.issued2015-12-18
dc.description.abstractAsthma is the most common chronic inflammatory disorder in children. The aetiology of asthma pathology is complex and highly heterogeneous, involving the interplay between genetic and environmental risk factors that is hypothesized to involve epigenetic processes. Our aim was to explore whether methylomic variation in early childhood is associated with discordance for asthma symptoms within monozygotic (MZ) twin pairs recruited from the Environmental Risk (E-Risk) longitudinal twin study. We also aimed to identify differences in DNA methylation that are associated with asthma that develops in childhood and persists into early adulthood as these may represent useful prognostic biomarkers. RESULTS: We examined genome-wide patterns of DNA methylation in buccal cell samples collected from 37 MZ twin pairs discordant for asthma at age 10. DNA methylation at individual CpG sites demonstrated significant variability within discordant MZ twin pairs with the top-ranked nominally significant differentially methylated position (DMP) located in the HGSNAT gene. We stratified our analysis by assessing DNA methylation differences in a sub-group of MZ twin pairs who remained persistently discordant for asthma at age 18. The top-ranked nominally significant DMP associated with persisting asthma is located in the vicinity of the HLX gene, which has been previously implicated in childhood asthma. CONCLUSIONS: We identified DNA methylation differences associated with childhood asthma in peripheral DNA samples from discordant MZ twin pairs. Our data suggest that differences in DNA methylation associated with childhood asthma which persists into early adulthood are distinct from those associated with asthma which remits.en_GB
dc.description.sponsorshipMedical Research Council (MRC)en_GB
dc.description.sponsorshipNational Institute of Child Health and Development (NICHD)en_GB
dc.description.sponsorshipAmerican Asthma Foundationen_GB
dc.description.sponsorshipMRC Centenary Awarden_GB
dc.identifier.citationClinical Epigenetics, 2015, Vol. 7: 130en_GB
dc.identifier.doi10.1186/s13148-015-0163-4
dc.identifier.grantnumberG1002190en_GB
dc.identifier.grantnumberHD077482en_GB
dc.identifier.grantnumberG9817803-E02/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/19115
dc.language.isoenen_GB
dc.publisherBioMed Centralen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26691723en_GB
dc.relation.urlhttp://www.clinicalepigeneticsjournal.com/content/7/1/130en_GB
dc.rights© 2015 Murphy et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_GB
dc.subjectAsthmaen_GB
dc.subjectDNA methylationen_GB
dc.subjectEpigeneticsen_GB
dc.subjectMonozygotic twinsen_GB
dc.titleMethylomic markers of persistent childhood asthma: a longitudinal study of asthma-discordant monozygotic twins.en_GB
dc.typeArticleen_GB
dc.date.available2016-01-05T12:42:44Z
dc.identifier.issn1868-7075
exeter.place-of-publicationGermany
dc.descriptionThis is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record.en_GB
dc.identifier.journalClinical Epigeneticsen_GB


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