dc.contributor.author | Dirks, ML | |
dc.contributor.author | Stephens, FB | |
dc.contributor.author | Jackman, SR | |
dc.contributor.author | Gordo, J | |
dc.contributor.author | Machin, D | |
dc.contributor.author | Pulsford, R | |
dc.contributor.author | van Loon, L | |
dc.contributor.author | Wall, B | |
dc.date.accessioned | 2018-04-19T09:06:09Z | |
dc.date.issued | 2018-04-18 | |
dc.description.abstract | The initial metabolic and molecular events that underpin disuse-induced skeletal muscle deconditioning, and the contribution of energy balance, remain to be investigated. Ten young, healthy males (age: 25 ± 1 y; BMI: 25.3 ± 0.8 kg m-2 ) underwent three 24 h laboratory-based experimental periods in a randomized, crossover manner: 1) controlled habitual physical activity with an energy-balanced diet (CON); 2) strict bed rest with a diet to maintain energy balance (BR-B); and 3) strict bed rest with a diet identical to CON, consequently resulting in positive energy balance. Continuous glucose monitoring was performed throughout each visit, with vastus lateralis muscle biopsies and an oral glucose tolerance test performed before and after. In parallel with muscle samples collected from a previous 7-day bed rest study, biopsies were used to examine expression of genes associated with the regulation of muscle mass and insulin sensitivity. A single day of bed rest, irrespective of energy balance, did not lead to overt changes in whole-body substrate oxidation, indices of insulin sensitivity (i.e. HOMA-IR (BR-B: from 2.7 ± 1.7 to 3.1 ± 1.5, P > 0.05), Matsuda (BR-B: from 5.9 ± 3.3 to 5.2 ± 2.9, P > 0.05)), or 24 h glycaemic control/variability compared to CON. Seven days of bed rest led to ∼30-55% lower expression of genes involved in insulin signalling, lipid storage/oxidation, and muscle protein breakdown, whereas no such changes were observed after one day of bed rest. In conclusion, more than one day of physical inactivity is required to observe the insulin resistance and robust skeletal muscle transcriptional responses associated with bed rest and consequent alterations in energy balance. | en_GB |
dc.description.sponsorship | BTW received internal funding from the College of Life and Environmental Sciences, University of Exeter, to support this project. None of the other authors received funding from any funding agency in the public, commercial or not-for-profit sectors to conduct this research. | en_GB |
dc.identifier.citation | First published: 15 April 2018 | en_GB |
dc.identifier.doi | 10.1113/EP086961 | |
dc.identifier.uri | http://hdl.handle.net/10871/32504 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.rights.embargoreason | Under embargo until 15th April 2019 in compliance with publisher policy. | en_GB |
dc.rights | This article is protected by copyright. All rights reserved. | en_GB |
dc.subject | disuse | en_GB |
dc.subject | glucose tolerance | en_GB |
dc.subject | insulin resistance | en_GB |
dc.subject | nutrition | en_GB |
dc.subject | transcriptional response | en_GB |
dc.title | A single day of bed rest, irrespective of energy balance, does not affect skeletal muscle gene expression or insulin sensitivity | en_GB |
dc.type | Article | en_GB |
dc.identifier.issn | 0958-0670 | |
dc.description | This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Experimental Physiology | en_GB |