dc.contributor.author | Holloway, GP | |
dc.contributor.author | Holwerda, AM | |
dc.contributor.author | Miotto, PM | |
dc.contributor.author | Dirks, ML | |
dc.contributor.author | Verdijk, LB | |
dc.contributor.author | van Loon, LJC | |
dc.date.accessioned | 2018-04-24T09:02:50Z | |
dc.date.issued | 2018-03-13 | |
dc.description.abstract | It remains unknown if mitochondrial bioenergetics are altered with aging in humans. We established an in vitro method to simultaneously determine mitochondrial respiration and H2O2emission in skeletal muscle tissue across a range of biologically relevant ADP concentrations. Using this approach, we provide evidence that, although the capacity for mitochondrial H2O2emission is not increased with aging, mitochondrial ADP sensitivity is impaired. This resulted in an increase in mitochondrial H2O2and the fraction of electron leak to H2O2, in the presence of virtually all ADP concentrations examined. Moreover, although prolonged resistance training in older individuals increased muscle mass, strength, and maximal mitochondrial respiration, exercise training did not alter H2O2emission rates in the presence of ADP, the fraction of electron leak to H2O2, or the redox state of the muscle. These data establish that a reduction in mitochondrial ADP sensitivity increases mitochondrial H2O2emission and contributes to age-associated redox stress. | en_GB |
dc.description.sponsorship | This work was funded by the Natural Sciences and Engineering Research Council of Canada (03656) and TI Food and Nutrition, a public-private partnership on precompetitive research in food and nutrition. | en_GB |
dc.identifier.citation | Vol. 22 (11), pp. 2837 - 2848 | en_GB |
dc.identifier.doi | 10.1016/j.celrep.2018.02.069 | |
dc.identifier.other | S2211-1247(18)30264-X | |
dc.identifier.uri | http://hdl.handle.net/10871/32576 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier (Cell Press) | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/29539414 | en_GB |
dc.rights | © 2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en_GB |
dc.subject | ADP | en_GB |
dc.subject | H(2)O(2) | en_GB |
dc.subject | ROS | en_GB |
dc.subject | aging | en_GB |
dc.subject | bioenergetics | en_GB |
dc.subject | exercise | en_GB |
dc.subject | mitochondria | en_GB |
dc.subject | muscle | en_GB |
dc.subject | resistance training | en_GB |
dc.subject | respiration | en_GB |
dc.title | Age-Associated Impairments in Mitochondrial ADP Sensitivity Contribute to Redox Stress in Senescent Human Skeletal Muscle. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-04-24T09:02:50Z | |
dc.identifier.issn | 2211-1247 | |
exeter.place-of-publication | United States | en_GB |
dc.description | This is the final version of the article. Available from Elsevier (Cell Press) via the DOI in this record. | en_GB |
dc.identifier.journal | Cell Reports | en_GB |