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dc.contributor.authorSong, Z
dc.contributor.authorMoore, DR
dc.contributor.authorHodson, N
dc.contributor.authorWard, C
dc.contributor.authorDent, JR
dc.contributor.authorO'Leary, MF
dc.contributor.authorShaw, AM
dc.contributor.authorHamilton, DL
dc.contributor.authorSarkar, S
dc.contributor.authorGangloff, Y-G
dc.contributor.authorHornberger, TA
dc.contributor.authorSpriet, LL
dc.contributor.authorHeigenhauser, GJ
dc.contributor.authorPhilp, A
dc.date.accessioned2019-03-06T09:37:24Z
dc.date.issued2017-07-10
dc.description.abstractThe mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.en_GB
dc.description.sponsorshipBiotechnology and Biological Science Research Council (BBSRC)en_GB
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC)en_GB
dc.description.sponsorshipChina Scholarship Councilen_GB
dc.description.sponsorshipNational Institute of Arthritis and Musculoskeletal and Skin Diseasesen_GB
dc.description.sponsorshipDepartment of Defenseen_GB
dc.identifier.citationVol. 7, Article number: 5028en_GB
dc.identifier.doi10.1038/s41598-017-05483-x
dc.identifier.grantnumberBB/L023547/1en_GB
dc.identifier.grantnumberAR057347en_GB
dc.identifier.grantnumberW81XWH-14–1–0105en_GB
dc.identifier.urihttp://hdl.handle.net/10871/36306
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017en_GB
dc.titleResistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscleen_GB
dc.typeArticleen_GB
dc.date.available2019-03-06T09:37:24Z
dc.identifier.issn2045-2322
exeter.article-numberARTN 5028en_GB
dc.descriptionThis is the final version. Available from the publisher via the DOI in this record.en_GB
dc.identifier.journalScientific Reportsen_GB
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_GB
dcterms.dateAccepted2019-06-19
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2017-07-10
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-03-06T09:32:28Z
refterms.versionFCDVoR
refterms.dateFOA2019-03-06T09:37:26Z
refterms.panelCen_GB


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Open Access This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or
format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this
article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the
material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the
copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2017
Except where otherwise noted, this item's licence is described as Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017