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dc.contributor.authorO'Leary, MF
dc.contributor.authorWallace, GR
dc.contributor.authorDavis, ET
dc.contributor.authorMurphy, DP
dc.contributor.authorNicholson, T
dc.contributor.authorBennett, AJ
dc.contributor.authorTsintzas, K
dc.contributor.authorJones, SW
dc.date.accessioned2019-03-06T10:31:07Z
dc.date.issued2018-10-18
dc.description.abstractAdiposity and adipokines are implicated in the loss of skeletal muscle mass with age and in several chronic disease states. The aim of this study was to determine the effects of human obese and lean subcutaneous adipose tissue secretome on myogenesis and metabolism in skeletal muscle cells derived from both young (18–30 yr) and elderly (>65 yr) individuals. Obese subcutaneous adipose tissue secretome impaired the myogenesis of old myoblasts but not young myoblasts. Resistin was prolifically secreted by obese subcutaneous adipose tissue and impaired myotube thickness and nuclear fusion by activation of the classical NFκB pathway. Depletion of resistin from obese adipose tissue secretome restored myogenesis. Inhibition of the classical NFκB pathway protected myoblasts from the detrimental effect of resistin on myogenesis. Resistin also promoted intramyocellular lipid accumulation in myotubes and altered myotube metabolism by enhancing fatty acid oxidation and increasing myotube respiration and ATP production. In conclusion, resistin derived from human obese subcutaneous adipose tissue impairs myogenesis of human skeletal muscle, particularly older muscle, and alters muscle metabolism in developing myotubes. These findings may have important implications for the maintenance of muscle mass in older people with chronic inflammatory conditions, or older people who are obese or overweight.en_GB
dc.description.sponsorshipWellcome Trusten_GB
dc.identifier.citationPublished online 18 October 2018en_GB
dc.identifier.doi10.1038/s41598-018-33840-x
dc.identifier.grantnumber102284/Z/13/Zen_GB
dc.identifier.urihttp://hdl.handle.net/10871/36313
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.rights© The Author(s). Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. en_GB
dc.subjectSarcopeniaen_GB
dc.subjectmyogenesisen_GB
dc.subjectresistinen_GB
dc.subjectobesityen_GB
dc.subjectadipokinesen_GB
dc.titleObese subcutaneous adipose tissue impairs human myogenesis, particularly in old skeletal muscle, via resistin-mediated activation of NFκBen_GB
dc.typeArticleen_GB
dc.date.available2019-03-06T10:31:07Z
dc.identifier.issn2045-2322
dc.descriptionThis is the author accepted manuscript. The final version is available from Nature Research via the DOI in this record.en_GB
dc.identifier.journalScientific Reportsen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2018-10-05
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2018-10-18
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-03-06T09:13:18Z
refterms.versionFCDAM
refterms.dateFOA2019-03-06T10:31:10Z
refterms.panelCen_GB
refterms.depositExceptionpublishedGoldOA
refterms.depositExceptionExplanationhttps://www.nature.com/articles/s41598-018-33840-x


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