Reduced skeletal muscle protein balance in paediatric Crohn's disease
dc.contributor.author | Davies, A | |
dc.contributor.author | Nixon, A | |
dc.contributor.author | Muhammed, R | |
dc.contributor.author | Tsintzas, K | |
dc.contributor.author | Kirkham, S | |
dc.contributor.author | Stephens, FB | |
dc.contributor.author | Moran, GW | |
dc.date.accessioned | 2019-07-04T08:03:57Z | |
dc.date.issued | 2019-05-25 | |
dc.description.abstract | Background & aims: An inability to respond to nutrition could be implicated in low muscle mass in Crohn's disease. We aim to determine skeletal muscle metabolic response to feeding in Crohn's disease and healthy volunteers. Methods: Twenty asymptomatic Crohn's disease participants (15.6 ± 0.5 yrs; BMI 20.6 ± 0.9 kg/m2); 9 with active disease (faecal calprotectin, 808 ± 225 ug/g and C-reactive protein, 2.2 ± 1.2 mg/dl), 11 in deep remission (faecal calprotectin, 61 ± 12 ug/g and C-reactive protein, 0.3 ± 0.2 mg/dl) and 9 matched healthy volunteers (16.0 ± 0.6 yrs; BMI 20.7 ± 0.6 kg/m2) were recruited. Participants had a dual energy X-ray absorptiometry scan, handgrip dynamometer test, wore a pedometer and completed a food diary. Arterialised hand and venous forearm blood samples were collected concurrently and brachial artery blood flow measured at baseline and every 20 min for 2 hrs after the ingestion of a standardised liquid meal. Net balance of branched chain amino acids (BCAA) and glucose were derived. Results: Controls had a positive mean BCAA balance. CD participants had an initial anabolic response to the meal, with increasing BCAA balance between t = 0 & t = 20, but returned to negative by t = 60. This was associated with reduced FFM z-scores in CD but not with insulin resistance or disease activity. Exploratory analyses suggest that negative postprandial BCAA response seen in CD is predominant in males (p = 0.049), with associated lower appendicular muscle mass (p = 0.034), higher muscle fatigue (p = 0.014) and reduced protein intake (p = 0.026). Conclusions: The inability to sustain a positive protein balance postprandially could provide an explanation for the reduced muscle mass seen in CD. Further mechanistic studies will be needed to confirm these findings. | en_GB |
dc.description.sponsorship | British Society of Paediatric Gastroenterology Hepatology and Nutrition-Core Development Award 2015 | en_GB |
dc.description.sponsorship | Nottingham University Hospitals Charity | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council | en_GB |
dc.description.sponsorship | National Institute of Health Research | en_GB |
dc.identifier.citation | Published online 25 May 2019 | en_GB |
dc.identifier.doi | 10.1016/j.clnu.2019.05.017 | |
dc.identifier.grantnumber | PP-G MORAN-APR15 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/37822 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.rights.embargoreason | Under embargo until 25 May 2020 in compliance with publisher policy. | en_GB |
dc.rights | © 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dc.subject | crohn's disease | en_GB |
dc.subject | Inflammatory bowel disease | en_GB |
dc.subject | Nutrition | en_GB |
dc.subject | Sarcopenia | en_GB |
dc.title | Reduced skeletal muscle protein balance in paediatric Crohn's disease | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-07-04T08:03:57Z | |
dc.identifier.issn | 0261-5614 | |
dc.description | This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record. | en_GB |
dc.identifier.journal | Clinical Nutrition | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2019-05-16 | |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-05-16 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-07-04T07:56:31Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2019-05-24T23:00:00Z | |
refterms.panel | C | en_GB |
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Except where otherwise noted, this item's licence is described as © 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/