Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise
dc.contributor.author | Wylie, LJ | |
dc.contributor.author | Park, JW | |
dc.contributor.author | Vanhatalo, A | |
dc.contributor.author | Kadach, S | |
dc.contributor.author | Black, MI | |
dc.contributor.author | Stoyanov, Z | |
dc.contributor.author | Schechter, AN | |
dc.contributor.author | Jones, AM | |
dc.contributor.author | Piknova, B | |
dc.date.accessioned | 2019-09-09T08:17:04Z | |
dc.date.issued | 2019-07-27 | |
dc.description.abstract | Rodent skeletal muscle contains a large store of nitrate that can be augmented by the consumption of dietary nitrate. This muscle nitrate reservoir has been found to be an important source of nitrite and nitric oxide (NO), via its reduction by tissue xanthine oxidoreductases (XOR). To explore if this pathway is also active in human skeletal muscle during exercise, and if it is sensitive to local nitrate availability, we assessed exercise-induced changes in muscle nitrate and nitrite concentrations in young healthy humans, under baseline conditions and following dietary nitrate consumption. We found that baseline nitrate and nitrite concentrations were far higher in muscle than in plasma (∼4-fold and ∼29-fold, respectively), and that the consumption of a single bolus of dietary nitrate (12.8 mmol) significantly elevated nitrate concentration in both plasma (∼19 fold) and muscle (∼5 fold). Consistent with these observations, and with previous suggestions of active muscle nitrate transport, we present Western blot data to show significant expression of the active nitrate/nitrite transporter, sialin, in human skeletal muscle. Furthermore, we report an exercise-induced reduction in human muscle nitrate concentration (by ∼39%), but only in the presence of an increased muscle nitrate store. Our results indicate that human skeletal muscle nitrate stores are sensitive to dietary nitrate intake and may contribute to NO generation during exercise. Together, these findings suggest that skeletal muscle plays an important role in the transport, storage and metabolism of nitrate in humans. This article is protected by copyright. All rights reserved. | en_GB |
dc.identifier.citation | Published online 27 July 2019 | en_GB |
dc.identifier.doi | 10.1113/JP278076 | |
dc.identifier.uri | http://hdl.handle.net/10871/38583 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley / Physiological Society | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/31350908 | en_GB |
dc.rights | © 2019 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | exercise | en_GB |
dc.subject | nitrate | en_GB |
dc.subject | nitric oxide | en_GB |
dc.subject | nitrite | en_GB |
dc.subject | skeletal muscle | en_GB |
dc.title | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-09-09T08:17:04Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.identifier.eissn | 1469-7793 | |
dc.identifier.journal | Journal of Physiology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-07-22 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-07-27 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-09-09T08:16:10Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-09-09T08:17:08Z | |
refterms.panel | C | en_GB |
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Except where otherwise noted, this item's licence is described as © 2019 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.