Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

DSpace/Manakin Repository

Open Research Exeter (ORE)

Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

Please use this identifier to cite or link to this item: http://hdl.handle.net/10036/12006

Share:                 



Title: Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.
Author: Mattingly, D
Martin, H
Tyler, C M
Citation: Journal of Clinical Pathology, 1993, 46(12):1109-1112
Journal: Journal of Clinical Pathology
Date Issued: 1993-12
URI: http://hdl.handle.net/10036/12006
Abstract: AIMS: To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism. METHODS: Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatography on silica gel and development of fluorescence on the plate with sulphuric acid. RESULTS: There was a linear correlation between the amount of aldosterone and the area under the peak on the chromatogram. The mean intra-assay and interassay coefficients of variation were 4.4% and 6.8%, respectively. The mean aldosterone excretion in 67 adults was 15.7 (SD 8.1) nmol/24 hours. The mean overnight excretion in 65 adults was 2.6 (1.4) nmol/8 hours. The method detected raised concentrations in patients with primary and secondary aldosteronism. CONCLUSIONS: This technique provides an accurate means of assaying urinary aldosterone. Overnight estimations seem to be as effective as 24 hour assays for identifying patients with hyperaldosteronism.
Type: Article
ISSN: 0021-97461472-4146
PubMed ID: 8282834
PubMed Central ID: 501721


Please note: Before reusing this item please check the rights under which it has been made available. Some items are restricted to non-commercial use. Please cite the published version where applicable.

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Browse

My Account

Local Links