Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

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Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

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dc.contributor.author Mattingly, D en_GB
dc.contributor.author Martin, H en_GB
dc.contributor.author Tyler, C M en_GB
dc.date.accessioned 2007-05-21T14:46:19Z en_GB
dc.date.accessioned 2011-01-25T10:08:11Z en_US
dc.date.accessioned 2013-03-20T17:08:22Z
dc.date.issued 1993-12 en_GB
dc.description.abstract AIMS: To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism. METHODS: Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatography on silica gel and development of fluorescence on the plate with sulphuric acid. RESULTS: There was a linear correlation between the amount of aldosterone and the area under the peak on the chromatogram. The mean intra-assay and interassay coefficients of variation were 4.4% and 6.8%, respectively. The mean aldosterone excretion in 67 adults was 15.7 (SD 8.1) nmol/24 hours. The mean overnight excretion in 65 adults was 2.6 (1.4) nmol/8 hours. The method detected raised concentrations in patients with primary and secondary aldosteronism. CONCLUSIONS: This technique provides an accurate means of assaying urinary aldosterone. Overnight estimations seem to be as effective as 24 hour assays for identifying patients with hyperaldosteronism. en_GB
dc.identifier.citation Journal of Clinical Pathology, 1993, 46(12):1109-1112 en_GB
dc.identifier.uri http://hdl.handle.net/10036/12006 en_GB
dc.language.iso en_US en_GB
dc.title Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry. en_GB
dc.type Article en_GB
dc.date.available 1993-12 en_GB
dc.date.available 2007-05-21T14:46:19Z en_GB
dc.date.available 2011-01-25T10:08:11Z en_US
dc.date.available 2013-03-20T17:08:22Z
dc.identifier.issn 0021-9746 en_GB
dc.identifier.issn 1472-4146 en_GB
dc.format.dig YES en_GB
dc.identifier.journal Journal of Clinical Pathology en_GB
dc.identifier.pmcid 501721 en_GB
dc.identifier.pmid 8282834 en_GB


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