Molecular Mechanisms of Neural Induction and Patterning in the Zebrafish Embryo

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Molecular Mechanisms of Neural Induction and Patterning in the Zebrafish Embryo

Show simple item record Pereira da Cruz, Carlos en_US 2011-11-18T09:20:51Z en_US 2013-03-21T10:33:24Z 2011-03-31 en_US
dc.description.abstract The brain is our most complex organ, with an estimated 1011 neurons. With the spinal cord, it forms the central nervous system which controls our movements and our senses, holds our memories and creates our thoughts. Because of this, neurodegenerative disorders can be extremely distressing and a thorough understanding of how the nervous system develops is essential if progress is to be made in finding ways to treat them. Critically, this includes understanding how the nervous system forms, i.e., the nature of the signals that promote neural identity (neural induction) and determine correct positional information (patterning). The zebrafish (Danio rerio) has become established as a model for embryological studies due to ease of experimental manipulation. Taking advantage of this, the aims of this PhD were to contribute to unravelling the molecular mechanisms of neural induction and patterning, using a variety of embryological and molecular methods. In the first project, functional analyses of the eve1 gene identified a key factor for posterior neural development. Eve1 was found to be a critical posteriorising factor, with an additional role in posterior neural induction. An outstanding question in neural induction is the relative contribution to this process of two key developmentally important signalling pathways, Bmp and Fgf. In the second project, differential analyses of maternal versus zygotic Bmp and Fgf signalling revealed crucial maternal roles for these two pathways in neural development as neural and epidermal capacitators. The results further suggested that Fgf signalling may be the critical neural inducer. Finally, as a third project, a zebrafish ectodermal explant assay was developed using the organiser-deficient ichabod mutant. The aim was to develop a system to analyse how key molecules directly affect ectoderm and neural development, free of mesoderm and endoderm influences, as signalling from these layers can directly or indirectly influence neural development. en_GB
dc.description.sponsorship BBSRC en_GB
dc.identifier.uri en_US
dc.language.iso en en_GB
dc.publisher University of Exeter en_GB
dc.rights.embargoreason To enable publication of the research en_GB
dc.subject Neural induction ichabod Bmp Fgf evx ectoderm en_GB
dc.title Molecular Mechanisms of Neural Induction and Patterning in the Zebrafish Embryo en_GB
dc.type Thesis or dissertation en_GB 2013-05-18T03:00:17Z
dc.contributor.advisor Kudoh, Tetsuhiro en_US
dc.publisher.department College of Life and Environmental Sciences en_GB
dc.publisher.department Biosciences en_GB
dc.type.degreetitle Doctor of Philosophy in Biological Sciences en_GB
dc.type.qualificationlevel Doctoral en_GB
dc.type.qualificationname PhD en_GB

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