dc.contributor.author | Goldsworthy, Michael John Howard | en_GB |
dc.date.accessioned | 2012-02-17T16:54:00Z | en_GB |
dc.date.accessioned | 2013-03-21T10:30:22Z | |
dc.date.issued | 2011-09 | en_GB |
dc.description.abstract | The continued depletion of oil reserves, a necessity for energy security
and the environmental importance of reducing greenhouse gas emissions has
prompted the industrial development of clean, non-conventional, renewable
fuels. Transport fuels are currently composed primarily of fossil-derived alkanes
and commonly contain a minor biofuel component of bioethanol in gasoline or
fatty acid methyl esters (FAMEs) in diesel. Increasing biofuel supply to satisfy
governmental targets imposes substantial technological constraints on
automobile engine manufacturers and raises controversies over arable land
usage. ‘Drop-in’, microbial, alkane biofuels are structurally identical to fossil
fuels and their production does not compete directly with arable farming,
thereby obviating the problems associated with the manufacture and
deployment of bioethanol and FAMEs.
This study investigated alkane biosynthesis in two non-photosynthetic
bacteria, Mycobacterium sp. NCIMB 10403 and Desulfovibrio desulfuricans, to
evaluate their potential for use as biocatalysts in the industrial manufacture of
‘drop-in’ biofuels. This study employed single- and two-dimensional gas
chromatography-mass spectrometry as a means to provide a rigorous analysis
of alkane biosynthesis in these bacteria. In addition, a microarray analysis was
used to develop an understanding of the genes potentially important in
regulating alkane production in D. desulfuricans.
In contrast to the original reports from the 1960’s, Mycobacterium sp.
NCIMB 10403 and D. desulfuricans NCIMB 8307 did not synthesise alkanes.
Alkane biosynthesis was confirmed in D. desulfuricans NCIMB 8326 although
the alkane quantities and carbon chain length distribution differed significantly
to those previously reported. The microarray data gave evidence to suggest
that the expression of genes encoding a long chain fatty acid-CoA ligase and an
aspartyl/glutamyl-tRNA amidotransferase may be important for regulating
alkane biosynthesis in D. desulfuricans. Furthermore, several genes encoding
hypothetical proteins were identified as being potentially involved directly in the
formation of alkanes. | en_GB |
dc.description.sponsorship | Royal Dutch Shell | en_GB |
dc.identifier.uri | http://hdl.handle.net/10036/3398 | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | University of Exeter | en_GB |
dc.rights.embargoreason | Maximum length of embargo requested for reasons of commercial confidentiality | en_GB |
dc.title | Hydrocarbon biosynthesis in Mycobacterium sp. NCIMB 10403 and Desulfovibrio desulfuricans | en_GB |
dc.type | Thesis or dissertation | en_GB |
dc.date.available | 2013-08-17T03:00:13Z | |
dc.contributor.advisor | Love, John | en_GB |
dc.publisher.department | Biosciences | en_GB |
dc.type.degreetitle | PhD in Biological Sciences | en_GB |
dc.type.qualificationlevel | Doctoral | en_GB |
dc.type.qualificationname | PhD | en_GB |