A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress
Bennett, Mark H
Mansfield, John W
University of Exeter
Copyright © 2008 Forcat et al; licensee BioMed Central Ltd.
We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.
Plant Methods 2008 4:16
PubMed Central ID