dc.contributor.author | Li, L | |
dc.contributor.author | Rose, P | |
dc.contributor.author | Tan, CH | |
dc.contributor.author | Parkinson, DB | |
dc.contributor.author | Moore, PK | |
dc.contributor.author | Whiteman, Matthew | |
dc.date.accessioned | 2013-06-25T13:35:59Z | |
dc.date.issued | 2010-05-15 | |
dc.description.abstract | The role of hydrogen sulfide (H(2)S) in inflammation is controversial, with both pro- and antiinflammatory effects documented. Many studies have used simple sulfide salts as the source of H(2)S, which give a rapid bolus of H(2)S in aqueous solutions and thus do not accurately reflect the enzymatic generation of H(2)S. We therefore compared the effects of sodium hydrosulfide and a novel slow-releasing H(2)S donor (GYY4137) on the release of pro- and antiinflammatory mediators in lipopolysaccharide (LPS)-treated murine RAW264.7 macrophages. For the first time, we show that GYY4137 significantly and concentration-dependently inhibits LPS-induced release of proinflammatory mediators such as IL-1beta, IL-6, TNF-alpha, nitric oxide (*NO), and PGE(2) but increased the synthesis of the antiinflammatory chemokine IL-10 through NF-kappaB/ATF-2/HSP-27-dependent pathways. In contrast, NaHS elicited a biphasic effect on proinflammatory mediators and, at high concentrations, increased the synthesis of IL-1beta, IL-6, NO, PGE(2) and TNF-alpha. This study clearly shows that the effects of H(2)S on the inflammatory process are complex and dependent not only on H(2)S concentration but also on the rate of H(2)S generation. This study may also explain some of the apparent discrepancies in the literature regarding the pro- versus antiinflammatory role of H(2)S. | en_GB |
dc.identifier.citation | Antioxidants and Redox Signaling, 2010, Vol. 12, Issue 10, pp. 1147 - 1154 | en_GB |
dc.identifier.doi | 10.1089/ars.2009.2899 | |
dc.identifier.uri | http://hdl.handle.net/10871/11383 | |
dc.language.iso | en | en_GB |
dc.publisher | Mary Ann Liebert | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/19769459 | en_GB |
dc.relation.url | http://online.liebertpub.com/doi/abs/10.1089/ars.2009.2899 | en_GB |
dc.subject | Activating Transcription Factor 2 | en_GB |
dc.subject | Air Pollutants | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Cell Line | en_GB |
dc.subject | HSP27 Heat-Shock Proteins | en_GB |
dc.subject | Hydrogen Sulfide | en_GB |
dc.subject | Inflammation Mediators | en_GB |
dc.subject | Lipopolysaccharides | en_GB |
dc.subject | Macrophages | en_GB |
dc.subject | Mice | en_GB |
dc.subject | Morpholines | en_GB |
dc.subject | NF-kappa B | en_GB |
dc.subject | Organothiophosphorus Compounds | en_GB |
dc.subject | Sulfides | en_GB |
dc.title | The effect of hydrogen sulfide donors on lipopolysaccharide-induced formation of inflammatory mediators in macrophages. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2013-06-25T13:35:59Z | |
exeter.place-of-publication | United States | |
dc.description | addresses: Peninsula Medical School, University of Exeter, St. Luke's Campus, Exeter, Devon, England. | en_GB |
dc.description | notes: PMCID: PMC2875982 | en_GB |
dc.description | This is a copy of an article published in the Antioxidants and Redox Signaling © 2010 copyright Mary Ann Liebert, Inc.; Antioxidants and Redox Signaling is available online at: http://online.liebertpub.com. | en_GB |
dc.identifier.journal | Antioxidants and Redox Signaling | en_GB |