Zinc can counteract selection for ciprofloxacin resistance
dc.contributor.author | Vos, M | |
dc.contributor.author | Sibleyras, L | |
dc.contributor.author | Lo, LK | |
dc.contributor.author | Hesse, E | |
dc.contributor.author | Gaze, W | |
dc.contributor.author | Klümper, U | |
dc.date.accessioned | 2020-07-03T13:55:35Z | |
dc.date.issued | 2020-02-27 | |
dc.description.abstract | Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-characterized, experimental studies exploring their combined effects on AMR evolution are rare. It has previously been demonstrated that fluoroquinolone antibiotics such as ciprofloxacin can chelate metal ions. To investigate how ciprofloxacin resistance is affected by the presence of metals, we quantified selection dynamics between a ciprofloxacin-susceptible and a ciprofloxacin-resistant Escherichia coli strain across a gradient of ciprofloxacin concentrations in presence and absence of zinc. The presence of zinc reduced growth of both strains, while ciprofloxacin inhibited exclusively the susceptible one. When present in combination zinc retained its inhibitory effect, while ciprofloxacin inhibition of the susceptible strain was reduced. Consequently, the minimal selective concentration for ciprofloxacin resistance increased up to five-fold in the presence of zinc. Environmental pollution usually comprises complex mixtures of antimicrobial agents. In addition to the usual focus on additive or synergistic interactions in complex selective mixtures, our findings highlight the importance of antagonistic selective interactions when considering resistance evolution. | en_GB |
dc.identifier.citation | Vol. 367 (3), article fnaa038 | en_GB |
dc.identifier.doi | 10.1093/femsle/fnaa038 | |
dc.identifier.uri | http://hdl.handle.net/10871/121770 | |
dc.language.iso | en | en_GB |
dc.publisher | Oxford University Press / Federation of European Microbiological Societies | en_GB |
dc.rights | © FEMS 2020. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | Antimicrobial resistance | en_GB |
dc.subject | Selection dynamics | en_GB |
dc.subject | Heavy metals | en_GB |
dc.subject | Chelation | en_GB |
dc.subject | Fluroquinolone | en_GB |
dc.subject | Antibiotic resistance | en_GB |
dc.title | Zinc can counteract selection for ciprofloxacin resistance | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-07-03T13:55:35Z | |
dc.identifier.issn | 0378-1097 | |
dc.description | This is the final version. Available on open access from Oxford University Press via the DOI in this record. | en_GB |
dc.identifier.eissn | 1574-6968 | |
dc.identifier.journal | FEMS Microbiology Letters | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2020-02-26 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2020-02-26 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-07-03T13:51:03Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-07-03T13:55:41Z | |
refterms.panel | A | en_GB |
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.