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dc.contributor.authorGrassi, L
dc.contributor.authorIzuogu, OG
dc.contributor.authorJorge, NAN
dc.contributor.authorSeyres, D
dc.contributor.authorBustamante, M
dc.contributor.authorBurden, F
dc.contributor.authorFarrow, S
dc.contributor.authorFarahi, N
dc.contributor.authorMartin, FJ
dc.contributor.authorFrankish, A
dc.contributor.authorMudge, JM
dc.contributor.authorKostadima, M
dc.contributor.authorPetersen, R
dc.contributor.authorLambourne, JJ
dc.contributor.authorRowlston, S
dc.contributor.authorMartin-Rendon, E
dc.contributor.authorClarke, L
dc.contributor.authorDownes, K
dc.contributor.authorEstivill, X
dc.contributor.authorFlicek, P
dc.contributor.authorMartens, JHA
dc.contributor.authorYaspo, M-L
dc.contributor.authorStunnenberg1, HG
dc.contributor.authorOuwehand, WH
dc.contributor.authorPassetti, F
dc.contributor.authorTurro, E
dc.contributor.authorFrontini, M
dc.date.accessioned2020-07-14T12:44:55Z
dc.date.issued2020-07-23
dc.description.abstractTranscriptional profiling of hematopoietic cell subpopulations has helped characterize the developmental stages of the hematopoietic system and the molecular bases of malignant and non-malignant blood diseases for the past three decades. Previously, only the genes targeted by expression microarrays could be profiled genome wide. High-throughput RNA sequencing (RNA-seq), however, encompasses a broader repertoire of RNA molecules, without restriction to previously annotated genes. We analysed the BLUEPRINT consortium RNA- seq data for mature hematopoietic cell types. The data comprised 90 total RNA-seq samples, each composed of one of 27 cell types, and 32 small RNA-seq samples, each composed of one of 11 cell types. We estimated gene and isoform expression levels for each cell type using existing annotations from Ensembl. We then used guided transcriptome assembly to discover unannotated transcripts. We identified hundreds of novel non-coding RNA genes and showed that the majority have cell type dependent expression. We also characterized the expression of circular RNAs and found that these are also cell type specific. These analyses refine the active transcriptional landscape of mature hematopoietic cells, highlight abundant genes and transcriptional isoforms for each blood cell type, and provide a valuable resource for researchers of hematological development and diseases.en_GB
dc.description.sponsorshipNational Institute for Health Research (NIHR)en_GB
dc.description.sponsorshipEuropean Union FP7en_GB
dc.description.sponsorshipBristol-Myers Squibben_GB
dc.description.sponsorshipBritish Heart Foundationen_GB
dc.description.sponsorshipMedical Research Council (MRC)en_GB
dc.description.sponsorshipWellcome Trusten_GB
dc.description.sponsorshipNational Institutes of Healthen_GB
dc.description.sponsorshipFundação Carlos Chagas Filho de Amparo à Pesquisado Estado do Rio de Janeiroen_GB
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasilen_GB
dc.description.sponsorshipRoyal Societyen_GB
dc.description.sponsorshipBritish Heart Foundationen_GB
dc.identifier.citationPublished online 23 July 2020
dc.identifier.doi10.3324/haematol.2019.238147
dc.identifier.grantnumber282510en_GB
dc.identifier.grantnumberWT108749/Z/15/Z)en_GB
dc.identifier.grantnumber2U41HG007234en_GB
dc.identifier.grantnumberE-26/203.229/2016en_GB
dc.identifier.grantnumberFS/18/53/33863en_GB
dc.identifier.urihttp://hdl.handle.net/10871/121930
dc.language.isoenen_GB
dc.publisherFerrata Storti Foundation / European Hematology Associationen_GB
dc.relation.urlhttps://blueprint.haem.cam.ac.uk/bloodatlas/en_GB
dc.rights© 2020, Ferrata Storti Foundation
dc.titleCell type specific novel lncRNAs in the BLUEPRINT hematopoietic transcriptomes atlasen_GB
dc.typeArticleen_GB
dc.date.available2020-07-14T12:44:55Z
dc.identifier.issn0390-6078
dc.descriptionThis is the author accepted manuscript. The final version is available on open access from the Ferrata Storti Foundation via the DOI in this recorden_GB
dc.descriptionData availability: data accessible via a web-based interface: https://blueprint.haem.cam.ac.uk/bloodatlas/en_GB
dc.identifier.journalHaematologicaen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2020-07-16
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2020-07-16
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2020-07-14T10:05:58Z
refterms.versionFCDAM
refterms.dateFOA2020-08-24T15:27:09Z
refterms.panelAen_GB


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