dc.contributor.author | Thines, M | |
dc.contributor.author | Sharma, R | |
dc.contributor.author | Rodenburg, SYA | |
dc.contributor.author | Gogleva, A | |
dc.contributor.author | Judelson, HS | |
dc.contributor.author | Xia, X | |
dc.contributor.author | van den Hoogen, J | |
dc.contributor.author | Kitner, M | |
dc.contributor.author | Klein, J | |
dc.contributor.author | Neilen, M | |
dc.contributor.author | de Ridder, D | |
dc.contributor.author | Seidl, MF | |
dc.contributor.author | van den Ackerveken, G | |
dc.contributor.author | Govers, F | |
dc.contributor.author | Schornack, S | |
dc.contributor.author | Studholme, DJ | |
dc.date.accessioned | 2020-07-16T08:15:40Z | |
dc.date.issued | 2020-04-01 | |
dc.description.abstract | Along with Plasmopara destructor, Peronosopora belbahrii has arguably been the economically most important newly emerging downy mildew pathogen of the past two decades. Originating from Africa, it has started devastating basil production throughout the world, most likely due to the distribution of infested seed material. Here, we present the genome of this pathogen and results from comparisons of its genomic features to other oomycetes. The assembly of the nuclear genome was around 35.4 Mbp in length, with an N50 scaffold length of around 248 kbp and an L50 scaffold count of 46. The circular mitochondrial genome consisted of around 40.1 kbp. From the repeat-masked genome, 9,049 protein-coding genes were predicted, out of which 335 were predicted to have extracellular functions, representing the smallest secretome so far found in peronosporalean oomycetes. About 16% of the genome consists of repetitive sequences, and, based on simple sequence repeat regions, we provide a set of microsatellites that could be used for population genetic studies of P. belbahrii. P. belbahrii has undergone a high degree of convergent evolution with other obligate parasitic pathogen groups, reflecting its obligate biotrophic lifestyle. Features of its secretome, signaling networks, and promoters are presented, and some patterns are hypothesized to reflect the high degree of host specificity in Peronospora species. In addition, we suggest the presence of additional virulence factors apart from classical effector classes that are promising candidates for future functional studies. | en_GB |
dc.description.sponsorship | Hesse’s Ministry of Higher Education, Research, and the Arts | en_GB |
dc.description.sponsorship | Wageningen University Fund | en_GB |
dc.description.sponsorship | Research Council Earth and Life Sciences of the Netherlands Organization of Scientific Research | en_GB |
dc.description.sponsorship | National Institute of Food and Agriculture (NIFA) | en_GB |
dc.description.sponsorship | National Science Foundation (NSF) | en_GB |
dc.description.sponsorship | Gatsby Charitable Foundation | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.identifier.citation | Vol. 33 (5), pp. 742 - 753 | en_GB |
dc.identifier.doi | 10.1094/MPMI-07-19-0211-R | |
dc.identifier.grantnumber | 863.15.005 | en_GB |
dc.identifier.grantnumber | 833.13.002 | en_GB |
dc.identifier.grantnumber | GAT3395/GLD | en_GB |
dc.identifier.grantnumber | UF160413 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/121967 | |
dc.language.iso | en | en_GB |
dc.publisher | American Phytopathological Society / International Society for Molecular Plant-Microbe Interactions | en_GB |
dc.relation.url | https://doi.org/10.4121/uuid:9b6e6aa0-b815-409f-9e96-04828b03290b | en_GB |
dc.rights.embargoreason | Under indefinite embargo due to publisher policy | en_GB |
dc.rights | © 2020 The American Phytopathological Society | en_GB |
dc.title | The Genome of Peronospora belbahrii Reveals High Heterozygosity, a Low Number of Canonical Effectors, and TC-Rich Promoters | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-07-16T08:15:40Z | |
dc.identifier.issn | 0894-0282 | |
dc.description | This is the author accepted manuscript. the final version is available from the American Phytopathological Society via the DOI in this record | en_GB |
dc.description | Data deposition:
The sequence data and annotations of the current study have been deposited at ENA under the
BioProject accession numbers PRJEB15119 and PRJEB20871. The KEGG Orthology hidden Markov
models are deposited at DOI 10.4121/uuid:9b6e6aa0-b815-409f-9e96-04828b03290b | en_GB |
dc.identifier.journal | Molecular Plant-Microbe Interactions | en_GB |
dc.rights.uri | http://www.rioxx.net/licenses/all-rights-reserved | en_GB |
dcterms.dateAccepted | 2020-02-23 | |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2020-04-01 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-07-16T08:09:36Z | |
refterms.versionFCD | AM | |
refterms.panel | A | en_GB |