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dc.contributor.authorYadav, B
dc.contributor.authorMora-Montes, HM
dc.contributor.authorWagener, J
dc.contributor.authorCunningham, I
dc.contributor.authorWest, L
dc.contributor.authorHaynes, K
dc.contributor.authorBrown, AJP
dc.contributor.authorGow, NAR
dc.date.accessioned2020-07-31T12:12:34Z
dc.date.issued2020-07-21
dc.description.abstractWe designed experiments to assess whether fungal cell wall mannans function as an immune shield or an immune agonist. Fungal cell wall β-(1,3)-glucan normally plays a major and dominant role in immune activation. The outer mannan layer has been variously described as an immune shield, because it has the potential to mask the underlying β-(1,3)-glucan, or an immune activator, as it also has the potential to engage with a wide range of mannose detecting PRRs. To resolve this conundrum we examined species-specific differences in host immune recognition in the och1Δ N-mannosylation-deficient mutant background in four species of yeast-like fungi. Irrespective of the fungal species, the cytokine response (TNFα and IL-6) induced by the och1Δ mutants in human monocytes was reduced compared to that of the wild type. In contrast, TNFα production induced by och1Δ was increased, relative to wild type, due to increased β-glucan exposure, when mouse or human macrophages were used. These observations suggest that N-mannan is not a major PAMP for macrophages and that in these cells mannan does shield the fungus from recognition of the inner cell wall β-glucan. However, Nmannan is a significant inducer of cytokine for monocytes. Therefore the metaphor of the fungal “mannan shield” can only be applied to some, but not all, myeloid cells used in immune profiling experiments of fungal speciesen_GB
dc.identifier.citationVol. 6, article 100042en_GB
dc.identifier.doi10.1016/j.tcsw.2020.100042
dc.identifier.urihttp://hdl.handle.net/10871/122274
dc.language.isoenen_GB
dc.publisherElsevieren_GB
dc.rights© 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).en_GB
dc.subjectFungal cell wallen_GB
dc.subjectN-linked mannosylationen_GB
dc.subjectβ-Glucanen_GB
dc.subjectMonocytesen_GB
dc.subjectMacrophagesen_GB
dc.titleDifferences in fungal immune recognition by monocytes and macrophages: N-mannan can be a shield or activator of immune recognitionen_GB
dc.typeArticleen_GB
dc.date.available2020-07-31T12:12:34Z
dc.identifier.issn2468-2330
exeter.article-number100042en_GB
dc.descriptionThis is the final version. Available on open access from Elsevier via the DOI in this record. en_GB
dc.identifier.journalThe Cell Surfaceen_GB
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/ en_GB
dcterms.dateAccepted2020-07-08
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2020-07-08
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2020-07-31T12:07:20Z
refterms.versionFCDVoR
refterms.dateFOA2020-07-31T12:12:38Z
refterms.panelUnspecifieden_GB


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© 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/BY-NC-ND/4.0/).
Except where otherwise noted, this item's licence is described as © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).