Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array
dc.contributor.author | Schuster, M | |
dc.contributor.author | Kilaru, S | |
dc.contributor.author | Fink, G | |
dc.contributor.author | Collemare, J | |
dc.contributor.author | Roger, Y | |
dc.contributor.author | Steinberg, G | |
dc.date.accessioned | 2020-12-07T13:16:53Z | |
dc.date.issued | 2011-10-01 | |
dc.description.abstract | The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde). Occasionally, EEs run up to 90 μm in one direction. The underlying MT array consists of unipolar MTs at both cell ends and antipolar bundles in the middle region of the cell. Cytoplasmic MT-organizing centers, labeled with a γ-tubulin ring complex protein, are distributed along the antipolar MTs but are absent from the unipolar regions. Dynein colocalizes with EEs for 10-20 μm after they have left the cell tip. Inactivation of temperature-sensitive dynein abolishes EE motility within the unipolar MT array, whereas long-range motility is not impaired. In contrast, kinesin-3 is continuously present, and its inactivation stops long-range EE motility. This indicates that both motors participate in EE motility, with dynein transporting the organelles through the unipolar MT array near the cell ends, and kinesin-3 taking over at the beginning of the medial antipolar MT array. The cooperation of both motors mediates EE movements over the length of the entire cell. © 2011 Schuster et al. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.identifier.citation | Vol. 22 (19), pp. 3645 - 3657 | en_GB |
dc.identifier.doi | 10.1091/mbc.E11-03-0217 | |
dc.identifier.grantnumber | BB/F022956/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/123936 | |
dc.language.iso | en | en_GB |
dc.publisher | American Society for Cell Biology | en_GB |
dc.rights | © 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). | en_GB |
dc.title | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-12-07T13:16:53Z | |
dc.identifier.issn | 1059-1524 | |
dc.description | This is the final version. Available from the American Society for Cell Biology via the DOI in this record | en_GB |
dc.identifier.journal | Molecular Biology of the Cell | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/3.0 | en_GB |
pubs.euro-pubmed-id | MED:21832152 | |
dcterms.dateAccepted | 2011-07-29 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2011-10-01 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-12-07T13:15:07Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-12-07T13:17:21Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).