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dc.contributor.authorSchuster, M
dc.contributor.authorKilaru, S
dc.contributor.authorFink, G
dc.contributor.authorCollemare, J
dc.contributor.authorRoger, Y
dc.contributor.authorSteinberg, G
dc.date.accessioned2020-12-07T13:16:53Z
dc.date.issued2011-10-01
dc.description.abstractThe polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde). Occasionally, EEs run up to 90 μm in one direction. The underlying MT array consists of unipolar MTs at both cell ends and antipolar bundles in the middle region of the cell. Cytoplasmic MT-organizing centers, labeled with a γ-tubulin ring complex protein, are distributed along the antipolar MTs but are absent from the unipolar regions. Dynein colocalizes with EEs for 10-20 μm after they have left the cell tip. Inactivation of temperature-sensitive dynein abolishes EE motility within the unipolar MT array, whereas long-range motility is not impaired. In contrast, kinesin-3 is continuously present, and its inactivation stops long-range EE motility. This indicates that both motors participate in EE motility, with dynein transporting the organelles through the unipolar MT array near the cell ends, and kinesin-3 taking over at the beginning of the medial antipolar MT array. The cooperation of both motors mediates EE movements over the length of the entire cell. © 2011 Schuster et al.en_GB
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC)en_GB
dc.identifier.citationVol. 22 (19), pp. 3645 - 3657en_GB
dc.identifier.doi10.1091/mbc.E11-03-0217
dc.identifier.grantnumberBB/F022956/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/123936
dc.language.isoenen_GB
dc.publisherAmerican Society for Cell Biologyen_GB
dc.rights© 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).en_GB
dc.titleKinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule arrayen_GB
dc.typeArticleen_GB
dc.date.available2020-12-07T13:16:53Z
dc.identifier.issn1059-1524
dc.descriptionThis is the final version. Available from the American Society for Cell Biology via the DOI in this recorden_GB
dc.identifier.journalMolecular Biology of the Cellen_GB
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0en_GB
pubs.euro-pubmed-idMED:21832152
dcterms.dateAccepted2011-07-29
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2011-10-01
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2020-12-07T13:15:07Z
refterms.versionFCDVoR
refterms.dateFOA2020-12-07T13:17:21Z
refterms.panelAen_GB


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© 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
Except where otherwise noted, this item's licence is described as © 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).