Correlating genotyping data of coxiella burnetii with genomic groups
dc.contributor.author | Hemsley, CM | |
dc.contributor.author | Essex‐lopresti, A | |
dc.contributor.author | Norville, IH | |
dc.contributor.author | Titball, RW | |
dc.date.accessioned | 2021-06-28T06:44:27Z | |
dc.date.issued | 2021-05-14 | |
dc.description.abstract | Coxiella burnetii is a zoonotic pathogen that resides in wild and domesticated animals across the globe and causes a febrile illness, Q fever, in humans. Several distinct genetic lineages or genomic groups have been shown to exist, with evidence for different virulence potential of these lineages. Multispacer Sequence Typing (MST) and Multiple‐Locus Variable number tandem repeat Analysis (MLVA) are being used to genotype strains. However, it is unclear how these typing schemes correlate with each other or with the classification into different genomic groups. Here, we created extensive databases for published MLVA and MST genotypes of C. burnetii and analysed the associated metadata, revealing associations between animal host and human disease type. We established a new classification scheme that assigns both MST and MLVA genotypes to a genomic group and which revealed additional sub‐lineages in two genomic groups. Finally, we report a novel, rapid genomotyping method for assigning an isolate into a genomic group based on the Cox51 spacer sequence. We conclude that by pooling and streamlining existing datasets, associations between genotype and clinical outcome or host source were identified, which in combination with our novel genomotyping method, should enable an estimation of the disease potential of new C. burnetii isolates. | en_GB |
dc.description.sponsorship | Defence Science and Technology Laboratories (DSTL) | en_GB |
dc.identifier.citation | Vol. 10 (5), article 604 | en_GB |
dc.identifier.doi | 10.3390/pathogens10050604 | |
dc.identifier.grantnumber | DSTLX-1000068994 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/126208 | |
dc.language.iso | en | en_GB |
dc.publisher | MDPI | en_GB |
dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_GB |
dc.subject | Coxiella burnetii | en_GB |
dc.subject | genotyping | en_GB |
dc.subject | MST | en_GB |
dc.subject | MLVA | en_GB |
dc.subject | genomic groups | en_GB |
dc.title | Correlating genotyping data of coxiella burnetii with genomic groups | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-06-28T06:44:27Z | |
dc.description | This is the final version. Available on open access from MDPI via the DOI in this record | en_GB |
dc.description | Data Availability Statement: All data are available in the Supplementary data file. | en_GB |
dc.identifier.eissn | 2076-0817 | |
dc.identifier.journal | Pathogens | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-05-12 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-05-14 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-06-27T08:33:12Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2021-06-28T06:44:33Z | |
refterms.panel | A | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).