Aspergillus fumigatus Acetate Utilization Impacts Virulence Traits and Pathogenicity
dc.contributor.author | Ries, LNA | |
dc.contributor.author | Alves de Castro, P | |
dc.contributor.author | Pereira Silva, L | |
dc.contributor.author | Valero, C | |
dc.contributor.author | dos Reis, TF | |
dc.contributor.author | Saborano, R | |
dc.contributor.author | Duarte, IF | |
dc.contributor.author | Persinoti, GF | |
dc.contributor.author | Steenwyk, JL | |
dc.contributor.author | Rokas, A | |
dc.contributor.author | Almeida, F | |
dc.contributor.author | Costa, JH | |
dc.contributor.author | Fill, T | |
dc.contributor.author | Sze Wah Wong, S | |
dc.contributor.author | Aimanianda, V | |
dc.contributor.author | Rodrigues, FJS | |
dc.contributor.author | Gonçales, RA | |
dc.contributor.author | Duarte-Oliveira, C | |
dc.contributor.author | Carvalho, A | |
dc.contributor.author | Goldman, GH | |
dc.date.accessioned | 2021-08-25T11:14:13Z | |
dc.date.issued | 2021-07-27 | |
dc.description.abstract | Aspergillus fumigatus is a major opportunistic fungal pathogen of immunocompromised and immunocompetent hosts. To successfully establish an infection, A. fumigatus needs to use host carbon sources, such as acetate, present in the body fluids and peripheral tissues. However, utilization of acetate as a carbon source by fungi in the context of infection has not been investigated. This work shows that acetate is metabolized via different pathways in A. fumigatus and that acetate utilization is under the regulatory control of a transcription factor (TF), FacB. A. fumigatus acetate utilization is subject to carbon catabolite repression (CCR), although this is only partially dependent on the TF and main regulator of CCR CreA. The available extracellular carbon source, in this case glucose and acetate, significantly affected A. fumigatus virulence traits such as secondary metabolite secretion and cell wall composition, with the latter having consequences for resistance to oxidative stress, antifungal drugs, and human neutrophil-mediated killing. Furthermore, deletion of facB significantly impaired the in vivo virulence of A. fumigatus in both insect and mammalian models of invasive aspergillosis. This is the first report on acetate utilization in A. fumigatus, and this work further highlights the importance of available host-specific carbon sources in shaping fungal virulence traits and subsequent disease outcome, and a potential target for the development of antifungal strategies. | en_GB |
dc.identifier.citation | Vol. 12 (4), article e01682-21 | en_GB |
dc.identifier.doi | 10.1128/mbio.01682-21 | |
dc.identifier.uri | http://hdl.handle.net/10871/126874 | |
dc.language.iso | en | en_GB |
dc.publisher | American Society for Microbiology | en_GB |
dc.rights | © 2021 Ries et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license: https://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | Aspergillus fumigatus Acetate Utilization Impacts Virulence Traits and Pathogenicity | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-08-25T11:14:13Z | |
dc.description | This is the final version. Available on open access from the American Society for Microbiology via the DOI in this record | en_GB |
dc.description | Data availability: The RNA-seq data set can be accessed at NCBI’s Short Read Archive under the Bioproject identifier (ID) PRJNA668271. | en_GB |
dc.identifier.eissn | 2150-7511 | |
dc.identifier.journal | mBio | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-06-10 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-07-27 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-08-25T11:11:54Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2021-08-25T11:14:25Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2021 Ries et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license: https://creativecommons.org/licenses/by/4.0/