Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate Lactate Release
dc.contributor.author | Vaccari Cardoso, B | |
dc.contributor.author | Barrera, I | |
dc.contributor.author | Mosienko, V | |
dc.contributor.author | Gourine, AV | |
dc.contributor.author | Kasparov, S | |
dc.contributor.author | Teschemacher, AG | |
dc.date.accessioned | 2021-09-02T10:07:02Z | |
dc.date.issued | 2021-08-10 | |
dc.description.abstract | Astrocytes support and modulate neuronal activity through the release of L-lactate. The suggested roles of astrocytic lactate in the brain encompass an expanding range of vital functions, including central control of respiration and cardiovascular performance, learning, memory, executive behaviour and regulation of mood. Studying the effects of astrocytic lactate requires tools that limit the release of lactate selectively from astrocytes. Here, we report the validation in vitro of novel molecular constructs derived from enzymes originally found in bacteria, that when expressed in astrocytes, interfere with lactate handling. When lactate 2-monooxygenase derived from M. smegmatis was specifically expressed in astrocytes, it reduced intracellular lactate pools as well as lactate release upon stimulation. D-lactate dehydrogenase derived from L. bulgaricus diverts pyruvate towards D-lactate production and release by astrocytes, which may affect signalling properties of lactate in the brain. Together with lactate oxidase, which we have previously described, this set of transgenic tools can be employed to better understand astrocytic lactate release and its role in the regulation of neuronal activity in different behavioural contexts | en_GB |
dc.description.sponsorship | British Heart Foundation | en_GB |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | en_GB |
dc.description.sponsorship | Academy of Medical Sciences | en_GB |
dc.identifier.citation | Vol. 11 (8), article 1056 | en_GB |
dc.identifier.doi | 10.3390/brainsci11081056 | |
dc.identifier.grantnumber | PG/18/8/33540 | en_GB |
dc.identifier.grantnumber | RG/19/5/34463 | en_GB |
dc.identifier.grantnumber | 206427/2014-0 | en_GB |
dc.identifier.grantnumber | SBF005\1102 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/126947 | |
dc.language.iso | en | en_GB |
dc.publisher | MDPI | en_GB |
dc.relation.url | https://www.addgene.org/ | en_GB |
dc.rights | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | en_GB |
dc.subject | astrocytes | en_GB |
dc.subject | lactate | en_GB |
dc.subject | gliotransmitter | en_GB |
dc.subject | viral vectors | en_GB |
dc.title | Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate Lactate Release | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-09-02T10:07:02Z | |
dc.description | This is the final version. Available on open access from MDPI via the DOI in this record | en_GB |
dc.description | Data Availability Statement: Data reported in this study are available upon request. Clones are available via addgene.org (accessed date 6 August 2021). | en_GB |
dc.identifier.eissn | 2076-3425 | |
dc.identifier.journal | Brain Sciences | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-08-06 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-08-10 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-09-02T10:06:33Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2021-09-02T10:07:10Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).