The epithelial splicing regulator ESRP2 is epigenetically repressed by DNA hypermethylation in Wilms tumour and acts as a tumour suppressor
dc.contributor.author | Legge, D | |
dc.contributor.author | Li, L | |
dc.contributor.author | Moriarty, W | |
dc.contributor.author | Lee, D | |
dc.contributor.author | Szemes, M | |
dc.contributor.author | Zahed, A | |
dc.contributor.author | Panousopoulos, L | |
dc.contributor.author | Chung, WY | |
dc.contributor.author | Aghabi, Y | |
dc.contributor.author | Barratt, J | |
dc.contributor.author | Williams, R | |
dc.contributor.author | Pritchard‐Jones, K | |
dc.contributor.author | Malik, KTA | |
dc.contributor.author | Oltean, S | |
dc.contributor.author | Brown, KW | |
dc.date.accessioned | 2021-09-17T11:34:49Z | |
dc.date.issued | 2021-09-14 | |
dc.description.abstract | Wilms tumour (WT), an embryonal kidney cancer, has been extensively characterised for genetic and epigenetic alterations, but a proportion of WTs still lack identifiable abnormalities. To uncover DNA methylation changes critical for WT pathogenesis, we compared the epigenome of fetal kidney with two WT cell lines, filtering our results to remove common cancer-associated epigenetic changes, and to enrich for genes involved in early kidney development. This identified four hypermethylated genes, of which ESRP2 (epithelial splicing regulatory protein 2) was the most promising for further study. ESRP2 was commonly repressed by DNA methylation in WT, and this occurred early in WT development (in nephrogenic rests). ESRP2 expression was reactivated by DNA methyltransferase inhibition in WT cell lines. When ESRP2 was overexpressed in WT cell lines, it inhibited cellular proliferation in vitro, and in vivo it suppressed tumour growth of orthotopic xenografts in nude mice. RNA-seq of the ESRP2-expressing WT cell lines identified several novel splicing targets. We propose a model in which epigenetic inactivation of ESRP2 disrupts the mesenchymal to epithelial transition in early kidney development to generate WT. | en_GB |
dc.description.sponsorship | Children's Cancer and Leukaemia Group | en_GB |
dc.description.sponsorship | Little Princess Trust | en_GB |
dc.description.sponsorship | Children with Cancer UK | en_GB |
dc.description.sponsorship | CLIC Sargent UK | en_GB |
dc.description.sponsorship | John James Bristol Foundation | en_GB |
dc.identifier.citation | Published online 14 September 2021 | en_GB |
dc.identifier.doi | 10.1002/1878-0261.13101 | |
dc.identifier.grantnumber | CCLGA 2017 11 | en_GB |
dc.identifier.grantnumber | 2012/135 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/127098 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley / Federation of European Biochemical Societies | en_GB |
dc.rights | © 2021. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | Wilms tumour | en_GB |
dc.subject | Epigenetics | en_GB |
dc.subject | DNA methylation | en_GB |
dc.subject | ESRP2 | en_GB |
dc.subject | MET | en_GB |
dc.title | The epithelial splicing regulator ESRP2 is epigenetically repressed by DNA hypermethylation in Wilms tumour and acts as a tumour suppressor | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-09-17T11:34:49Z | |
dc.identifier.issn | 1574-7891 | |
dc.description | This is the author accepted manuscript. The final version is available on open access from Wiley via the DOI in this record | en_GB |
dc.identifier.journal | Molecular Oncology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
exeter.funder | ::Children's Cancer and Leukaemia Group | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-09-16T12:01:04Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2021-09-17T11:34:55Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2021. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.