The polygonal cell shape and surface protein layer of anaerobic methane-oxidizing Methylomirabilis ianthanidiphila bacteria
dc.contributor.author | Gambelli, L | |
dc.contributor.author | Mesman, R | |
dc.contributor.author | Versantvoort, W | |
dc.contributor.author | Diebolder, CA | |
dc.contributor.author | Engel, A | |
dc.contributor.author | Evers, W | |
dc.contributor.author | Jetten, MSM | |
dc.contributor.author | Pabst, M | |
dc.contributor.author | Daum, B | |
dc.contributor.author | van Niftrik, L | |
dc.date.accessioned | 2021-12-15T10:23:46Z | |
dc.date.issued | 2021-12-01 | |
dc.date.updated | 2021-12-15T09:56:08Z | |
dc.description.abstract | Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative surface protein layer (S-layer) was observed as the outermost cell layer of these bacteria. We hypothesized that this S-layer is the determining factor for their polygonal cell shape. Therefore, we enriched the S-layer from M. lanthanidiphila cells and through LC-MS/MS identified a 31 kDa candidate S-layer protein, mela_00855, which had no homology to any other known protein. Antibodies were generated against a synthesized peptide derived from the mela_00855 protein sequence and used in immunogold localization to verify its identity and location. Both on thin sections of M. lanthanidiphila cells and in negative-stained enriched S-layer patches, the immunogold localization identified mela_00855 as the S-layer protein. Using electron cryo-tomography and sub-tomogram averaging of S-layer patches, we observed that the S-layer has a hexagonal symmetry. Cryo-tomography of whole cells showed that the S-layer and the outer membrane, but not the peptidoglycan layer and the cytoplasmic membrane, exhibited the polygonal shape. Moreover, the S-layer consisted of multiple rigid sheets that partially overlapped, most likely giving rise to the unique polygonal cell shape. These characteristics make the S-layer of M. lanthanidiphila a distinctive and intriguing case to study. | en_GB |
dc.description.sponsorship | European Commission | en_GB |
dc.description.sponsorship | European Research Council | en_GB |
dc.description.sponsorship | OCW/NWO Gravitation grant | en_GB |
dc.description.sponsorship | European Research Council | en_GB |
dc.description.sponsorship | Netherlands Organisation for Scientific Research (NWO) | en_GB |
dc.format.extent | 766527- | |
dc.identifier.citation | Vol. 12, article 766527 | en_GB |
dc.identifier.doi | https://doi.org/10.3389/fmicb.2021.766527 | |
dc.identifier.grantnumber | 803894 | en_GB |
dc.identifier.grantnumber | ERC-AG 339880 | en_GB |
dc.identifier.grantnumber | SIAM 024002002 | en_GB |
dc.identifier.grantnumber | ERC-AG 669371 | en_GB |
dc.identifier.grantnumber | VI.Vidi.192.001 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/128127 | |
dc.identifier | ORCID: 0000-0002-3767-264X (Daum, Bertram) | |
dc.language.iso | en | en_GB |
dc.publisher | Frontiers Media SA | en_GB |
dc.rights | Copyright © 2021 Gambelli, Mesman, Versantvoort, Diebolder, Engel, Evers, Jetten, Pabst, Daum and van Niftrik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en_GB |
dc.subject | Methylomirabilis | en_GB |
dc.subject | NC10 phylum | en_GB |
dc.subject | anaerobic methane oxidation | en_GB |
dc.subject | S-layer | en_GB |
dc.subject | cell shape | en_GB |
dc.subject | cryo-tomography | en_GB |
dc.subject | sub-tomogram averaging | en_GB |
dc.title | The polygonal cell shape and surface protein layer of anaerobic methane-oxidizing Methylomirabilis ianthanidiphila bacteria | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-12-15T10:23:46Z | |
dc.identifier.issn | 1664-302X | |
dc.description | This is the final version. Available from Frontiers Media via the DOI in this record. | en_GB |
dc.description | The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: ProteomeXchange, PXD029319; EMDB, EMD-13672 and EMD-13670; and EMPIAR, EMPIAR-10822 and EMPIAR-10829 | en_GB |
dc.identifier.eissn | 1664-302X | |
dc.identifier.journal | Frontiers in Microbiology | en_GB |
dc.relation.ispartof | Frontiers in Microbiology, 12 | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-11-08 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-12-01 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-12-15T10:16:07Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2021-12-15T10:25:55Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2021-12-01 |
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Except where otherwise noted, this item's licence is described as Copyright © 2021 Gambelli, Mesman, Versantvoort, Diebolder, Engel, Evers, Jetten,
Pabst, Daum and van Niftrik. This is an open-access article distributed under the
terms of the Creative Commons Attribution License (CC BY). The use, distribution
or reproduction in other forums is permitted, provided the original author(s) and
the copyright owner(s) are credited and that the original publication in this journal
is cited, in accordance with accepted academic practice. No use, distribution or
reproduction is permitted which does not comply with these terms.