Fc-conjugated C-type lectin receptors: Tools for understanding host-pathogen interactions
dc.contributor.author | Willment, JA | |
dc.date.accessioned | 2022-02-07T14:46:22Z | |
dc.date.issued | 2021-10-28 | |
dc.date.updated | 2022-02-07T14:00:38Z | |
dc.description.abstract | The use of soluble fusion proteins of pattern recognition receptors (PRRs) used in the detection of exogenous and endogenous ligands has helped resolve the roles of PRRs in the innate immune response to pathogens, how they shape the adaptive immune response, and function in maintaining homeostasis. Using the immunoglobulin (Ig) crystallizable fragment (Fc) domain as a fusion partner, the PRR fusion proteins are soluble, stable, easily purified, have increased affinity due to the Fc homodimerization properties, and consequently have been used in a wide range of applications such as flow cytometry, screening of protein and glycan arrays, and immunofluorescent microscopy. This review will predominantly focus on the recognition of pathogens by the cell membrane-expressed glycan-binding proteins of the C-type lectin receptor (CLR) subgroup of PRRs. PRRs bind to conserved pathogen-associated molecular patterns (PAMPs), such as glycans, usually located within or on the outer surface of the pathogen. Significantly, many glycans structures are identical on both host and pathogen (e.g. the Lewis (Le) X glycan), allowing the use of Fc CLR fusion proteins with known endogenous and/or exogenous ligands as tools to identify pathogen structures that are able to interact with the immune system. Screens of highly purified pathogen-derived cell wall components have enabled identification of many unique PAMP structures recognized by CLRs. This review highlights studies using Fc CLR fusion proteins, with emphasis on the PAMPs found in fungi, bacteria, viruses, and parasites. The structure and unique features of the different CLR families is presented using examples from a broad range of microbes whenever possible. | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.format.medium | Print-Electronic | |
dc.identifier.citation | Published online 28 October 2021 | en_GB |
dc.identifier.doi | https://doi.org/10.1111/mmi.14837 | |
dc.identifier.grantnumber | MR/N006364/2 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/128731 | |
dc.identifier | ORCID: 0000-0002-7040-0857 (Willment, Janet A) | |
dc.identifier | ScopusID: 6602846387 (Willment, Janet A) | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/34709692 | en_GB |
dc.rights | © 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commo ns Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | detection | en_GB |
dc.subject | glycans | en_GB |
dc.subject | immunlogy | en_GB |
dc.subject | microbial structure | en_GB |
dc.subject | microbial-cell interaction | en_GB |
dc.title | Fc-conjugated C-type lectin receptors: Tools for understanding host-pathogen interactions | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2022-02-07T14:46:22Z | |
dc.identifier.issn | 0950-382X | |
exeter.place-of-publication | England | |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.description | Data availability statement: Data sharing is not applicable to this article as no new data were created or analysed in this study. | en_GB |
dc.identifier.eissn | 1365-2958 | |
dc.identifier.journal | Molecular Microbiology | en_GB |
dc.relation.ispartof | Mol Microbiol | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-10-25 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-10-28 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2022-02-07T14:41:51Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2022-02-07T14:46:35Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2021-10-28 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commo ns Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.