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An archaellum filament composed of two alternating subunits

dc.contributor.authorGambelli, L
dc.contributor.authorIsupov, MN
dc.contributor.authorConners, R
dc.contributor.authorMcLaren, M
dc.contributor.authorBellack, A
dc.contributor.authorGold, V
dc.contributor.authorRachel, R
dc.contributor.authorDaum, B
dc.date.accessioned2022-07-01T10:00:11Z
dc.date.issued2022-02-07
dc.date.updated2022-07-01T09:39:16Z
dc.description.abstractArchaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament's flexibility.en_GB
dc.description.sponsorshipEuropean Research Councilen_GB
dc.description.sponsorshipWellcome Trusten_GB
dc.description.sponsorshipDeutsche Forschungsgemeinschaften_GB
dc.description.sponsorshipUniversity of Exeteren_GB
dc.format.extent710-
dc.format.mediumElectronic
dc.identifier.citationVol. 13, article 710en_GB
dc.identifier.doihttps://doi.org/10.1038/s41467-022-28337-1
dc.identifier.grantnumber803894en_GB
dc.identifier.grantnumber210363/Z/18/Zen_GB
dc.identifier.grantnumberWI 731/10-1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/130112
dc.identifierORCID: 0000-0001-6842-4289 (Isupov, Michail N)
dc.identifierORCID: 0000-0002-8653-1771 (Conners, Rebecca)
dc.identifierResearcherID: C-2608-2013 (Conners, Rebecca)
dc.identifierORCID: 0000-0002-6908-0745 (Gold, Vicki)
dc.identifierORCID: 0000-0002-3767-264X (Daum, Bertram)
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/35132062en_GB
dc.rights© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.en_GB
dc.subjectArchaeal Proteinsen_GB
dc.subjectBinding Sitesen_GB
dc.subjectCryoelectron Microscopyen_GB
dc.subjectFlagellaen_GB
dc.subjectFlagellinen_GB
dc.subjectGlycosylationen_GB
dc.subjectMetalsen_GB
dc.subjectMethanocaldococcusen_GB
dc.subjectModels, Molecularen_GB
dc.subjectProtein Multimerizationen_GB
dc.subjectProtein Subunitsen_GB
dc.subjectArchaeal biologyen_GB
dc.subjectCryoelectron microscopyen_GB
dc.titleAn archaellum filament composed of two alternating subunitsen_GB
dc.typeArticleen_GB
dc.date.available2022-07-01T10:00:11Z
dc.identifier.issn2041-1723
exeter.article-number710
exeter.place-of-publicationEngland
dc.descriptionThis is the final version. Available from Nature Research via the DOI in this record. en_GB
dc.identifier.eissn2041-1723
dc.identifier.journalNature Communicationsen_GB
dc.relation.ispartofNat Commun, 13(1)
dc.rights.urihttp://creativecommons.org/ licenses/by/4.0/en_GB
dcterms.dateAccepted2022-01-20
dc.rights.licenseCC BY
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2022-02-07
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2022-07-01T09:54:16Z
refterms.versionFCDVoR
refterms.dateFOA2022-07-01T10:00:17Z
refterms.panelAen_GB
refterms.dateFirstOnline2022-02-07


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© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
Except where otherwise noted, this item's licence is described as © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.