Non-antibiotic pharmaceuticals promote conjugative plasmid transfer at a community-wide level
dc.contributor.author | Wang, Y | |
dc.contributor.author | Yu, Z | |
dc.contributor.author | Ding, P | |
dc.contributor.author | Lu, J | |
dc.contributor.author | Klümper, U | |
dc.contributor.author | Murray, AK | |
dc.contributor.author | Gaze, WH | |
dc.contributor.author | Guo, J | |
dc.date.accessioned | 2022-08-25T10:10:45Z | |
dc.date.issued | 2022-08-12 | |
dc.date.updated | 2022-08-25T09:44:48Z | |
dc.description.abstract | BACKGROUND: Horizontal gene transfer (HGT) plays a critical role in the spread of antibiotic resistance and the evolutionary shaping of bacterial communities. Conjugation is the most well characterized pathway for the spread of antibiotic resistance, compared to transformation and transduction. While antibiotics have been found to induce HGT, it remains unknown whether non-antibiotic pharmaceuticals can facilitate conjugation at a microbial community-wide level. RESULTS: In this study, we demonstrate that several commonly consumed non-antibiotic pharmaceuticals (including carbamazepine, ibuprofen, naproxen and propranolol), at environmentally relevant concentrations (0.5 mg/L), can promote the conjugative transfer of IncP1-α plasmid-borne antibiotic resistance across entire microbial communities. The over-generation of reactive oxygen species in response to these non-antibiotic pharmaceuticals may contribute to the enhanced conjugation ratios. Cell sorting and 16S rRNA gene amplicon sequencing analyses indicated that non-antibiotic pharmaceuticals modulate transconjugant microbial communities at both phylum and genus levels. Moreover, microbial uptake ability of the IncP1-α plasmid was also upregulated under non-antibiotic pharmaceutical exposure. Several opportunistic pathogens, such as Acinetobacter and Legionella, were more likely to acquire the plasmid conferring multidrug resistance. CONCLUSIONS: Considering the high possibility of co-occurrence of pathogenic bacteria, conjugative IncP1-α plasmids and non-antibiotic pharmaceuticals in various environments (e.g., activated sludge systems), our findings illustrate the potential risk associated with increased dissemination of antibiotic resistance promoted by non-antibiotic pharmaceuticals in complex environmental settings. Video abstract. | en_GB |
dc.description.sponsorship | Australian Research Council (ARC) | en_GB |
dc.description.sponsorship | UQ Foundation Research | en_GB |
dc.description.sponsorship | China Scholarship Council | en_GB |
dc.description.sponsorship | European Union | en_GB |
dc.description.sponsorship | BMBF | en_GB |
dc.format.extent | 124- | |
dc.format.medium | Electronic | |
dc.identifier.citation | Vol. 10, article 124 | en_GB |
dc.identifier.doi | https://doi.org/10.1186/s40168-022-01314-y | |
dc.identifier.grantnumber | DP220101526 | en_GB |
dc.identifier.grantnumber | FT170100196 | en_GB |
dc.identifier.grantnumber | 1822 | en_GB |
dc.identifier.grantnumber | 01LC1904A | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/130504 | |
dc.identifier | ORCID: 0000-0002-1388-754X (Murray, Aimee K) | |
dc.language.iso | en | en_GB |
dc.publisher | BMC | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/35953866 | en_GB |
dc.rights | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | en_GB |
dc.title | Non-antibiotic pharmaceuticals promote conjugative plasmid transfer at a community-wide level | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2022-08-25T10:10:45Z | |
dc.identifier.issn | 2049-2618 | |
exeter.article-number | 124 | |
exeter.place-of-publication | England | |
dc.description | This is the final version. Available on open access from BMC via the DOI in this record | en_GB |
dc.description | Availability of data and materials; The datasets generated during and/or analyzed during the current study are available in NCBI under accession number PRJNA746678. | en_GB |
dc.identifier.eissn | 2049-2618 | |
dc.identifier.journal | Microbiome | en_GB |
dc.relation.ispartof | Microbiome, 10(1) | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2022-06-13 | |
dc.rights.license | CC BY | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2022-08-12 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2022-08-25T10:07:57Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2022-08-25T10:13:20Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2022-08-12 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.