The host phylogeny determines viral infectivity and replication across Staphylococcus host species
dc.contributor.author | Walsh, SK | |
dc.contributor.author | Imrie, RM | |
dc.contributor.author | Matuszewska, M | |
dc.contributor.author | Paterson, GK | |
dc.contributor.author | Weinert, LA | |
dc.contributor.author | Hadfield, JD | |
dc.contributor.author | Buckling, A | |
dc.contributor.author | Longdon, B | |
dc.date.accessioned | 2023-05-31T13:14:00Z | |
dc.date.issued | 2023-06-08 | |
dc.date.updated | 2023-05-31T12:47:31Z | |
dc.description.abstract | Virus host shifts, where a virus transmits to and infects a novel host species, are a major source of emerging infectious disease. Genetic similarity between eukaryotic host species has been shown to be an important determinant of the outcome of virus host shifts, but it is unclear if this is the case for prokaryotes where anti-virus defences can be transmitted by horizontal gene transfer and evolve rapidly. Here, we measure the susceptibility of 64 strains of Staphylococcaceae bacteria (48 strains of Staphylococcus aureus and 16 non-S. aureus species spanning 2 genera) to the bacteriophage ISP, which is currently under investigation for use in phage therapy. Using three methods – plaque assays, optical density (OD) assays, and quantitative (q)PCR – we find that the host phylogeny explains a large proportion of the variation in susceptibility to ISP across the host panel. These patterns were consistent in models of only S. aureus strains and models with a single representative from each Staphylococcaceae species, suggesting that these phylogenetic effects are conserved both within and among host species. We find positive correlations between susceptibility assessed using OD and qPCR and variable correlations between plaque assays and either OD or qPCR, suggesting that plaque assays alone may be inadequate to assess host range. Furthermore, we demonstrate that the phylogenetic relationships between bacterial hosts can generally be used to predict the susceptibility of bacterial strains to phage infection when the susceptibility of closely related hosts is known, although this approach produced large prediction errors in multiple strains where phylogeny was uninformative. Together, our results demonstrate the ability of bacterial host evolutionary relatedness to explain differences in susceptibility to phage infection, with implications for the development of ISP both as a phage therapy treatment and as an experimental system for the study of virus host shifts. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | |
dc.description.sponsorship | Medical Research Council (MRC) | |
dc.description.sponsorship | Raymond and Beverly Sackler Fund | |
dc.description.sponsorship | Wellcome Trust | |
dc.description.sponsorship | Royal Society | |
dc.description.sponsorship | Natural Environment Research Council (NERC) | |
dc.identifier.citation | Vol. 19(6), article e1011433 | en_GB |
dc.identifier.doi | 10.1371/journal.ppat.1011433 | |
dc.identifier.grantnumber | BB/M009122/1 | |
dc.identifier.grantnumber | 109385/Z/15/Z | |
dc.identifier.grantnumber | NE/P000924/1 | |
dc.identifier.grantnumber | NE/S000771/1 | |
dc.identifier.grantnumber | NE/V012347/1 | |
dc.identifier.grantnumber | 109356/Z/15/Z | |
dc.identifier.uri | http://hdl.handle.net/10871/133260 | |
dc.language.iso | en | en_GB |
dc.publisher | Public Library of Science | en_GB |
dc.relation.url | https://doi.org/10.6084/m9.figshare.21642209.v1 | |
dc.rights | © 2023 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.title | The host phylogeny determines viral infectivity and replication across Staphylococcus host species | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2023-05-31T13:14:00Z | |
dc.identifier.issn | 1553-7366 | |
dc.description | This is the final version. Available on open access from the Public Library of Science via the DOI in this record | en_GB |
dc.description | Data Availability: All data files and R scripts used in this study are available in an online repository: https://doi.org/10.6084/m9.figshare.21642209.v1 | |
dc.identifier.eissn | 1553-7374 | |
dc.identifier.journal | PLoS Pathogens | en_GB |
dc.relation.ispartof | PLoS Pathogens | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2023-05-18 | |
dcterms.dateSubmitted | 2022-12-08 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2023-05-18 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2023-05-31T12:47:34Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2023-07-06T14:40:05Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2023 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.