The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm.
dc.contributor.author | Louis, M | |
dc.contributor.author | Tahrioui, A | |
dc.contributor.author | Lendon, CJ | |
dc.contributor.author | Clamens, T | |
dc.contributor.author | Leprince, J | |
dc.contributor.author | Lefranc, B | |
dc.contributor.author | Kipnis, E | |
dc.contributor.author | Grandjean, T | |
dc.contributor.author | Bouffartigues, E | |
dc.contributor.author | Barreau, M | |
dc.contributor.author | Defontaine, F | |
dc.contributor.author | Cornelis, P | |
dc.contributor.author | Feuilloley, MGJ | |
dc.contributor.author | Harmer, NJ | |
dc.contributor.author | Chevalier, S | |
dc.contributor.author | Lesouhaitier, O | |
dc.date.accessioned | 2023-07-20T10:09:42Z | |
dc.date.issued | 2023-05-24 | |
dc.date.updated | 2023-07-20T08:46:43Z | |
dc.description.abstract | Biofilms are highly tolerant to antimicrobials and host immune defense, enabling pathogens to thrive in hostile environments. The diversity of microbial biofilm infections requires alternative and complex treatment strategies. In a previous work we demonstrated that the human Atrial Natriuretic Peptide (hANP) displays a strong anti-biofilm activity toward Pseudomonas aeruginosa and that the binding of hANP by the AmiC protein supports this effect. This AmiC sensor has been identified as an analog of the human natriuretic peptide receptor subtype C (h-NPRC). In the present study, we evaluated the anti-biofilm activity of the h-NPRC agonist, osteocrin (OSTN), a hormone that displays a strong affinity for the AmiC sensor at least in vitro. Using molecular docking, we identified a pocket in the AmiC sensor that OSTN reproducibly docks into, suggesting that OSTN might possess an anti-biofilm activity as well as hANP. This hypothesis was validated since we observed that OSTN dispersed established biofilm of P. aeruginosa PA14 strain at the same concentrations as hANP. However, the OSTN dispersal effect is less marked than that observed for the hANP (-61% versus -73%). We demonstrated that the co-exposure of P. aeruginosa preformed biofilm to hANP and OSTN induced a biofilm dispersion with a similar effect to that observed with hANP alone suggesting a similar mechanism of action of these two peptides. This was confirmed by the observation that OSTN anti-biofilm activity requires the activation of the complex composed by the sensor AmiC and the regulator AmiR of the ami pathway. Using a panel of both P. aeruginosa laboratory reference strains and clinical isolates, we observed that the OSTN capacity to disperse established biofilms is highly variable from one strain to another. Taken together, these results show that similarly to the hANP hormone, OSTN has a strong potential to be used as a tool to disperse P. aeruginosa biofilms. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Region Normandy | en_GB |
dc.description.sponsorship | European and Regional Development Fund | en_GB |
dc.description.sponsorship | InterReg IVA PeReNE project | en_GB |
dc.description.sponsorship | Normandy Valorisation | en_GB |
dc.format.extent | 100131- | |
dc.format.medium | Electronic-eCollection | |
dc.identifier.citation | Vol. 5, article 100131 | en_GB |
dc.identifier.doi | https://doi.org/10.1016/j.bioflm.2023.100131 | |
dc.identifier.uri | http://hdl.handle.net/10871/133628 | |
dc.identifier | ORCID: 0000-0002-4073-0505 (Harmer, Nicholas J) | |
dc.identifier | ScopusID: 6603323310 (Harmer, Nicholas J) | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/37252226 | en_GB |
dc.rights | © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). | en_GB |
dc.subject | Bacterial adaptation | en_GB |
dc.subject | Bacterial sensor | en_GB |
dc.subject | Biofilm | en_GB |
dc.subject | Natriuretic peptides | en_GB |
dc.subject | Osteocrin | en_GB |
dc.subject | h-NPR-C | en_GB |
dc.title | The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2023-07-20T10:09:42Z | |
dc.identifier.issn | 2590-2075 | |
exeter.article-number | 100131 | |
exeter.place-of-publication | Netherlands | |
dc.description | This is the final version. Available from Elsevier via the DOI in this record. | en_GB |
dc.description | Data availability: Data will be made available on request. | en_GB |
dc.identifier.journal | Biofilm | en_GB |
dc.relation.ispartof | Biofilm, 5 | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2023-05-18 | |
dc.rights.license | CC BY-NC-ND | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2023-05-24 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2023-07-20T10:03:49Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2023-07-20T10:09:50Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2023-05-24 |
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Except where otherwise noted, this item's licence is described as © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).