Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae
dc.contributor.author | Edwards, S | |
dc.contributor.author | De Fine Licht, HH | |
dc.date.accessioned | 2024-01-10T14:53:54Z | |
dc.date.issued | 2023-12-13 | |
dc.date.updated | 2024-01-10T12:02:05Z | |
dc.description.abstract | Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation.•E. muscae isolation and culturing in vitro.•Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica).•Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver. | en_GB |
dc.description.sponsorship | European Union Horizon 2020 | en_GB |
dc.description.sponsorship | Independent Research Fund Denmark | en_GB |
dc.description.sponsorship | Carlsberg Foundation | en_GB |
dc.identifier.citation | Vol. 12, article 102523 | en_GB |
dc.identifier.doi | https://doi.org/10.1016/j.mex.2023.102523 | |
dc.identifier.grantnumber | 859850 | en_GB |
dc.identifier.grantnumber | 8049–00086A | en_GB |
dc.identifier.grantnumber | CF20–0609 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/134977 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38179068 | en_GB |
dc.rights | © 2023 Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | en_GB |
dc.subject | Behavioural manipulation model | en_GB |
dc.subject | Diptera | en_GB |
dc.subject | Host-parasite interaction | en_GB |
dc.subject | In vitro | en_GB |
dc.subject | In vivo | en_GB |
dc.subject | Insect pathogenic fungus | en_GB |
dc.subject | Isolation and culturing of Entomophthora muscae | en_GB |
dc.title | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-01-10T14:53:54Z | |
dc.identifier.issn | 2215-0161 | |
exeter.article-number | 102523 | |
exeter.place-of-publication | Netherlands | |
dc.description | This is the final version. Available on open access from Elsevier via the DOI in this record. | en_GB |
dc.description | Data availability: No data was used for the research described in the article. | en_GB |
dc.identifier.journal | MethodsX | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2023-12-12 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2023-12-13 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-01-10T14:49:20Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2024-01-10T14:53:58Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2023-12-13 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2023 Published by Elsevier B.V. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/)