Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure.
dc.contributor.author | Nikolic, N | |
dc.contributor.author | Anagnostidis, V | |
dc.contributor.author | Tiwari, A | |
dc.contributor.author | Chait, R | |
dc.contributor.author | Gielen, F | |
dc.date.accessioned | 2024-02-02T13:57:49Z | |
dc.date.issued | 2023-11-21 | |
dc.date.updated | 2024-02-02T12:43:28Z | |
dc.description.abstract | An alarming rise in antimicrobial resistance worldwide has spurred efforts into the search for alternatives to antibiotic treatments. The use of bacteriophages, bacterial viruses harmless to humans, represents a promising approach with potential to treat bacterial infections (phage therapy). Recent advances in microscopy-based single-cell techniques have allowed researchers to develop new quantitative methodologies for assessing the interactions between bacteria and phages, especially the ability of phages to eradicate bacterial pathogen populations and to modulate growth of both commensal and pathogen populations. Here we combine droplet microfluidics with fluorescence time-lapse microscopy to characterize the growth and lysis dynamics of the bacterium Escherichia coli confined in droplets when challenged with phage. We investigated phages that promote lysis of infected E. coli cells, specifically, a phage species with DNA genome, T7 (Escherichia virus T7) and two phage species with RNA genomes, MS2 (Emesvirus zinderi) and Qβ (Qubevirus durum). Our microfluidic trapping device generated and immobilized picoliter-sized droplets, enabling stable imaging of bacterial growth and lysis in a temperature-controlled setup. Temporal information on bacterial population size was recorded for up to 25 h, allowing us to determine growth rates of bacterial populations and helping us uncover the extent and speed of phage infection. In the long-term, the development of novel microfluidic single-cell and population-level approaches will expedite research towards fundamental understanding of the genetic and molecular basis of rapid phage-induced lysis and eco-evolutionary aspects of bacteria-phage dynamics, and ultimately help identify key factors influencing the success of phage therapy. | en_GB |
dc.description.sponsorship | Wellcome Trust | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.description.sponsorship | BBSRC-funded South West Biosciences Doctoral Training Partnership | en_GB |
dc.format.extent | 1260196- | |
dc.format.medium | Electronic-eCollection | |
dc.identifier.citation | Vol. 14, article 1260196 | en_GB |
dc.identifier.doi | https://doi.org/10.3389/fmicb.2023.1260196 | |
dc.identifier.grantnumber | WT105618MA | en_GB |
dc.identifier.grantnumber | BB/T011777/1 | en_GB |
dc.identifier.grantnumber | RGS/R2/192377 | en_GB |
dc.identifier.grantnumber | 2578821 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/135231 | |
dc.identifier | ORCID: 0000-0001-9068-6090 (Nikolic, Nela) | |
dc.identifier | ORCID: 0000-0003-0876-3187 (Chait, Remy) | |
dc.identifier | ScopusID: 16232464200 (Chait, Remy) | |
dc.identifier | ORCID: 0000-0003-0604-7224 (Gielen, Fabrice) | |
dc.language.iso | en | en_GB |
dc.publisher | Frontiers Media | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38075890 | en_GB |
dc.relation.url | https://openwetware.org/wiki/ DropBase:Anchored_droplet | en_GB |
dc.rights | © 2023 Nikolic, Anagnostidis, Tiwari, Chait and Gielen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en_GB |
dc.subject | Escherichia coli | en_GB |
dc.subject | bacterial growth | en_GB |
dc.subject | bacterial population | en_GB |
dc.subject | droplet microfluidics | en_GB |
dc.subject | phage | en_GB |
dc.subject | phage therapy | en_GB |
dc.subject | phage-induced lysis | en_GB |
dc.subject | time-lapse microscopy | en_GB |
dc.title | Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-02-02T13:57:49Z | |
dc.identifier.issn | 1664-302X | |
exeter.article-number | ARTN 1260196 | |
exeter.place-of-publication | Switzerland | |
dc.description | Data availability statement: The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding authors. Design files for microfluidic devices are deposited on DropBase (https://openwetware.org/wiki/ DropBase:Anchored_droplet). | en_GB |
dc.identifier.journal | Frontiers in Microbiology | en_GB |
dc.relation.ispartof | Front Microbiol, 14 | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2023-10-23 | |
dc.rights.license | CC BY | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2023-11-21 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-02-02T13:53:10Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2024-02-02T13:57:53Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2023-11-21 |
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