A cytochrome P450 insecticide detoxification mechanism is not conserved across the Megachilidae family of bees
dc.contributor.author | Hayward, A | |
dc.contributor.author | Hunt, BJ | |
dc.contributor.author | Haas, J | |
dc.contributor.author | Bushnell-Crowther, E | |
dc.contributor.author | Troczka, BJ | |
dc.contributor.author | Pym, A | |
dc.contributor.author | Beadle, K | |
dc.contributor.author | Field, J | |
dc.contributor.author | Nelson, DR | |
dc.contributor.author | Nauen, R | |
dc.contributor.author | Bass, C | |
dc.date.accessioned | 2024-03-05T10:25:19Z | |
dc.date.issued | 2023-12-06 | |
dc.date.updated | 2024-03-04T18:38:30Z | |
dc.description.abstract | Recent work has demonstrated that many bee species have specific cytochrome P450 enzymes (P450s) that can efficiently detoxify certain insecticides. The presence of these P450s, belonging or closely related to the CYP9Q subfamily (CYP9Q-related), is generally well conserved across the diversity of bees. However, the alfalfa leafcutter bee, Megachile rotundata, lacks CYP9Q-related P450s and is 170-2500 times more sensitive to certain insecticides than bee pollinators with these P450s. The extent to which these findings apply to other Megachilidae bee species remains uncertain. To address this knowledge gap, we sequenced the transcriptomes of four Megachile species and leveraged the data obtained, in combination with publicly available genomic data, to investigate the evolution and function of P450s in the Megachilidae. Our analyses reveal that several Megachilidae species, belonging to the Lithurgini, Megachilini and Anthidini tribes, including all species of the Megachile genus investigated, lack CYP9Q-related genes. In place of these genes Megachile species have evolved phylogenetically distinct CYP9 genes, the CYP9DM lineage. Functional expression of these P450s from M. rotundata reveal they lack the capacity to metabolize the neonicotinoid insecticides thiacloprid and imidacloprid. In contrast, species from the Osmiini and Dioxyini tribes of Megachilidae have CYP9Q-related P450s belonging to the CYP9BU subfamily that are able to detoxify thiacloprid. These findings provide new insight into the evolution of P450s that act as key determinants of insecticide sensitivity in bees and have important applied implications for pesticide risk assessment. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Bayer AG | en_GB |
dc.identifier.citation | Vol. 17(1), article e13625 | en_GB |
dc.identifier.doi | https://doi.org/10.1111/eva.13625 | |
dc.identifier.grantnumber | BB/V004093/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/135475 | |
dc.identifier | ORCID: 0000-0003-0663-4031 (Field, Jeremy) | |
dc.identifier | ORCID: 0000-0002-2590-1492 (Bass, Chris) | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38283601 | en_GB |
dc.rights | © 2023 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | ecotoxicology | en_GB |
dc.subject | gene structure and function | en_GB |
dc.subject | molecular evolution | en_GB |
dc.title | A cytochrome P450 insecticide detoxification mechanism is not conserved across the Megachilidae family of bees | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-03-05T10:25:19Z | |
dc.identifier.issn | 1752-4571 | |
exeter.place-of-publication | England | |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.description | Data availability statement: Raw sequence data and assemblies have been submitted to NCBI Short Read Archive and Transcriptome Shotgun Assembly databases under BioProject PRJNA962714. All other relevant data are provided in the supplementary information. | en_GB |
dc.identifier.eissn | 1752-4571 | |
dc.identifier.journal | Evolutionary Applications | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2023-11-19 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2023-12-06 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-03-05T10:23:13Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2024-03-05T10:27:05Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2023-12-06 |
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Except where otherwise noted, this item's licence is described as © 2023 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.