UDP-glycosyltransferases act as key determinants of host plant range in generalist and specialist Spodoptera species.
dc.contributor.author | Wang, H | |
dc.contributor.author | Song, J | |
dc.contributor.author | Hunt, BJ | |
dc.contributor.author | Zuo, K | |
dc.contributor.author | Zhou, H | |
dc.contributor.author | Hayward, A | |
dc.contributor.author | Li, B | |
dc.contributor.author | Xiao, Y | |
dc.contributor.author | Geng, X | |
dc.contributor.author | Bass, C | |
dc.contributor.author | Zhou, S | |
dc.date.accessioned | 2024-05-16T12:19:47Z | |
dc.date.issued | 2024-05-07 | |
dc.date.updated | 2024-05-16T10:03:22Z | |
dc.description.abstract | Phytophagous insects have evolved sophisticated detoxification systems to overcome the antiherbivore chemical defenses produced by many plants. However, how these biotransformation systems differ in generalist and specialist insect species and their role in determining insect host plant range remains an open question. Here, we show that UDP-glucosyltransferases (UGTs) play a key role in determining the host range of insect species within the Spodoptera genus. Comparative genomic analyses of Spodoptera species that differ in host plant breadth identified a relatively conserved number of UGT genes in generalist species but high levels of UGT gene pseudogenization in the specialist Spodoptera picta. CRISPR-Cas9 knockouts of the three main UGT gene clusters of Spodoptera frugiperda revealed that UGT33 genes play an important role in allowing this species to utilize the poaceous plants maize, wheat, and rice, while UGT40 genes facilitate utilization of cotton. Further functional analyses in vivo and in vitro identified the UGT SfUGT33F32 as the key mechanism that allows generalist S. frugiperda to detoxify the benzoxazinoid DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), a potent insecticidal phytotoxin produced by poaceous plants. However, while this detoxification capacity is conserved in several generalist Spodoptera species, Spodoptera picta, which specializes on Crinum plants, is unable to detoxify DIMBOA due to a nonfunctionalizing mutation in SpUGT33F34. Collectively, these findings provide insight into the role of insect UGTs in host plant adaptation, the mechanistic basis of evolutionary transitions between generalism and specialism and offer molecular targets for controlling a group of notorious insect pests. | en_GB |
dc.description.sponsorship | National Natural Science Foundation of China | en_GB |
dc.description.sponsorship | Key Research and Development Project of Henan Province | en_GB |
dc.description.sponsorship | Natural Science Foundation of Henan Province | en_GB |
dc.description.sponsorship | Young Elite Scientists Sponsorship Program by Henan Association for Science and Technology | en_GB |
dc.description.sponsorship | National Natural Science Foundation of China | en_GB |
dc.description.sponsorship | Internationalization training of high-level talents in Henan Province. | en_GB |
dc.identifier.citation | Vol. 121, No. 19, article e2402045121 | en_GB |
dc.identifier.doi | https://doi.org/10.1073/pnas.2402045121 | |
dc.identifier.grantnumber | U2004142 | en_GB |
dc.identifier.grantnumber | 221111112200 | en_GB |
dc.identifier.grantnumber | 232300421107 | en_GB |
dc.identifier.grantnumber | 2022HYTP038 | en_GB |
dc.identifier.grantnumber | 32102213 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/135959 | |
dc.identifier | ORCID: 0000-0003-3825-9723 (Hunt, Benjamin J) | |
dc.identifier | ORCID: 0000-0002-2590-1492 (Bass, Chris) | |
dc.language.iso | en | en_GB |
dc.publisher | National Academy of Sciences | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38683998 | en_GB |
dc.rights | © 2024 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY). | en_GB |
dc.subject | DIMBOA | en_GB |
dc.subject | Spodoptera pests | en_GB |
dc.subject | UDP-glucosyltransferases | en_GB |
dc.subject | host adaptation | en_GB |
dc.subject | xenobiotic detoxification | en_GB |
dc.title | UDP-glycosyltransferases act as key determinants of host plant range in generalist and specialist Spodoptera species. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-05-16T12:19:47Z | |
dc.identifier.issn | 0027-8424 | |
exeter.place-of-publication | United States | |
dc.description | This is the final version. Available on open access from National Academy of Sciences via the DOI in this record. | en_GB |
dc.description | Data, Materials, and Software Availability: The raw sequencing data for S. picta has been deposited at National Center for Biotechnology Information under the BioProject PRJNA1073306 (55). The genome assembly and annotation of S. picta and the transcriptomic raw data for different tissues of S. frugiperda have been deposited at DRYAD (56). All study data are included in the article and/or supporting information. | en_GB |
dc.identifier.eissn | 1091-6490 | |
dc.identifier.journal | Proceedings of the National Academy of Sciences (PNAS) | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2024-03-13 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2024-05-07 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-05-16T12:14:07Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2024-05-16T12:19:58Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2024-04-29 | |
exeter.rights-retention-statement | No |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2024 the Author(s). Published by PNAS.
This open access article is distributed under Creative
Commons Attribution License 4.0 (CC BY).