Bulk segregant mapping and transcriptome analyses reveal the molecular mechanisms of spinetoram resistance in Spodoptera frugiperda
dc.contributor.author | Kanno, RH | |
dc.contributor.author | Nascimento, ARB | |
dc.contributor.author | Monteiro, CP | |
dc.contributor.author | Amaral, FSA | |
dc.contributor.author | Singh, KS | |
dc.contributor.author | Troczka, BJ | |
dc.contributor.author | Bass, C | |
dc.contributor.author | Cônsoli, FL | |
dc.contributor.author | Omoto, C | |
dc.date.accessioned | 2024-05-20T12:32:58Z | |
dc.date.issued | 2024-04-20 | |
dc.date.updated | 2024-05-20T09:07:56Z | |
dc.description.abstract | The evolution of resistance to insecticides poses a significant threat to pest management programs. Understanding the molecular mechanisms underlying insecticide resistance is essential to design sustainable pest control and resistance management programs. The fall armyworm, Spodoptera frugiperda, is an important insect pest of many crops and has a remarkable ability to evolve resistance to insecticides. In this study, we employed bulk segregant analysis (BSA) combined with DNA and RNA sequencing to characterize the molecular basis of spinetoram resistance in S. frugiperda. Analysis of genomic data derived from spinetoram selected and unselected bulks and the spinetoram-resistant and susceptible parental strains led to the identification of a three-nucleotide deletion in the gene encoding the nicotinic acetylcholine receptor α6 subunit (nAChR α6). Transcriptome profiling identified the upregulation of few genes encoding detoxification enzymes associated with spinetoram resistance. Thus, spinetoram resistance in S. frugiperda appears to be mediated mainly by target site insensitivity with a minor role of detoxification enzymes. Our findings provide insight into the mechanisms underpinning resistance to spinetoram in S. frugiperda and will inform the development of strategies to control this highly damaging, globally distributed crop pest. | en_GB |
dc.description.sponsorship | São Paulo Research Foundation (FAPESP) | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Brazilian National Council for Scientific and Technological Development (CNPq) | en_GB |
dc.format.extent | 105921- | |
dc.identifier.citation | Vol. 202, article 105921 | en_GB |
dc.identifier.doi | https://doi.org/10.1016/j.pestbp.2024.105921 | |
dc.identifier.grantnumber | 2019/06217–8 | en_GB |
dc.identifier.grantnumber | 2019/17215–6 | en_GB |
dc.identifier.grantnumber | 2018/21155–6 | en_GB |
dc.identifier.grantnumber | BB/R022623/1 | en_GB |
dc.identifier.grantnumber | 2017/50455–5 | en_GB |
dc.identifier.grantnumber | BB/S018719/1 | en_GB |
dc.identifier.grantnumber | 2018/21155–6 | en_GB |
dc.identifier.grantnumber | 141089/2018–0 | en_GB |
dc.identifier.grantnumber | 314160/2020–5 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/135980 | |
dc.identifier | ORCID: 0000-0002-2590-1492 (Bass, Chris) | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.rights.embargoreason | Under embargo until 20 April 2025 in compliance with publisher policy | en_GB |
dc.rights | © 2024 Elsevier Inc. This version is made available under the CC-BY-NC-ND licence: https://creativecommons.org/by-nc-nd/4.0 | en_GB |
dc.subject | Fall armyworm | en_GB |
dc.subject | Spinosyn | en_GB |
dc.subject | DNA-Seq | en_GB |
dc.subject | RNA-Seq | en_GB |
dc.subject | BSA | en_GB |
dc.title | Bulk segregant mapping and transcriptome analyses reveal the molecular mechanisms of spinetoram resistance in Spodoptera frugiperda | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-05-20T12:32:58Z | |
dc.identifier.issn | 0048-3575 | |
exeter.article-number | 105921 | |
dc.description | This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record | en_GB |
dc.description | Data availability: Illumina data will be publicly available at the NCBI BioProject PRJNA1103734, PRJNA1104133, PRJNA110461 and, PRJNA1104299. The data that support the findings of this study are available from the corresponding author upon reasonable request. | en_GB |
dc.identifier.journal | Pesticide Biochemistry and Physiology | en_GB |
dc.relation.ispartof | Pesticide Biochemistry and Physiology | |
dc.rights.uri | https://creativecommons.org/by-nc-nd/4.0 | en_GB |
dcterms.dateAccepted | 2024-04-18 | |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2024-04-20 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-05-20T12:27:25Z | |
refterms.versionFCD | AM | |
refterms.panel | A | en_GB |
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