Investigation of Microsporidia and Protist Symbionts in Reared Insect Hosts - Insights from Nanopore Sequencing and Host-Parasite Interactions

Abstract

The mass rearing of insects for food, feed, and research is a rapidly growing industry, presenting unique challenges related to the health and management of reared insect populations. This thesis synthesised research on protist and microsporidian parasites that affect industrially reared insects, highlighting the need for a comprehensive understanding of these organisms’ impacts on insect health and rearing practices. By utilising advanced molecular tools and innovative methodologies, this work aimed to improve diagnostic capabilities, enhance parasite control strategies, and deepen the understanding of host-parasite interactions. Through detailed studies on various parasites, including microsporidia and gregarines, and the use of third-generation sequencing technologies, this research contributed valuable insights into maintaining insect health and optimising rearing practices in the insect farming industry.